Results 1 to 10 of about 31,371 (190)

Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applications [PDF]

Frontiers in Veterinary Science
The well-known technique of microtomy, which is an essential cutting tool, was first developed for light and transmission electron microscope uses, but it is currently also utilized to prepare specimens for atomic force microscopy (AFM), ion microscopy ...
Yasmin M. Abd El-Aziz, Mohammed S. Sobh, Hebatallah M. Saad, Menna H. E. Morsy, Ehab El-Haroun, Kasim Sakran Abass, Zulhisyam Abdul Kari, Roshmon Thomas Mathew, Moaheda E. H. Eissa, El-Sayed Hemdan Eissa   +9 more
doaj   +2 more sources

Protocol for the establishment and morphological characterization of long-term cultivated murine cerebral organoids [PDF]

STAR Protocols
Summary: Murine cerebral organoids provide a rapid and reproducible in vitro system that recapitulates key aspects of neurogenesis. While human cerebral organoid protocols are well established, methods for non-human models remain limited.
Issam El-Debs, Michael R. Knittler, Thomas C. Mettenleiter, John O. Mason, Julia Sehl-Ewert   +4 more
doaj   +2 more sources

Serial section videography (SSV): A low‐cost protocol for generating 3D reconstructions of internal plant structure [PDF]

Applications in Plant Sciences
Premise Analyzing structural changes along the length of an organ provides insight into its development. However, traditional histological methods are limited by intensive procedures and size restrictions.
Jacob S. Suissa, Giselle Reyes
doaj   +2 more sources

The mechanics of paraffin sectioning by the microtome [PDF]

The Anatomical Record, 1942
No Abstract.
exaly   +3 more sources

A Protocol for Laser-Assisted Microdissection and tRF & tiRNA Sequencing in Lung Adenocarcinoma [PDF]

Bio-Protocol
Laser-assisted microdissection (LAM) coupled with next-generation sequencing technologies offers a powerful approach to dissecting the complex cellular heterogeneity within lung adenocarcinoma (LUAD) tumors.
Zi Wang, Qinglin Wang, Lin Xu, Qixing Mao, Feng Jiang   +4 more
doaj   +2 more sources

Cyclooxygenase-2 and von Willebrand factor—an immunohistochemical study of the equine foot with and without laminitis, post-mortem perfused with paraffin oil [PDF]

Frontiers in Veterinary Science
ObjectiveEquine laminitis is a complex and potentially fatal disease characterized by severe vascular and inflammatory alterations within the equine foot.
Bianca A. Underberg, Bianca A. Underberg, Elke Van der Vekens, Barbara Drews, Sabine Kaessmeyer   +4 more
doaj   +2 more sources

Targeted 2D histology and ultrastructural bone analysis based on 3D microCT anatomical locations

MethodsX, 2021
Histological processing of mineralised tissue (e.g. bone) allows examining the anatomy of cells and tissues as well as the material properties of the tissue. However, resin-embedding offers limited control over the specimen position for cutting. Moreover,
I. Moreno-Jiménez, D.S. Garske, C.A. Lahr, D.W. Hutmacher, A. Cipitria   +4 more
doaj   +1 more source

Shoot Development through Modified Transverse Thin Cell Layer (tTCL) Culture of Phalaenopsis Hybrid Protocorms

Horticulturae, 2022
This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella.
Kuo-Chin Lo, Jualang Azlan Gansau, Chia-Hung Shih, Chien-Yuan Kao   +3 more
doaj   +1 more source

The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning

Plant Methods, 2023
Background Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements.
David J. Thomas, Jordan Rainbow, Laura E. Bartley   +2 more
doaj   +1 more source

A SIMPLE NEW MICROTOME FOR ULTRATHIN SECTIONING [PDF]

Journal of Histochemistry & Cytochemistry, 1954
A simple easily-constructed microtome capable of cutting thin sections of the order of 300 Å in thickness for electron microscope study is described. Design criteria for such microtomes, as well as some of the problems in thin sectioning are discussed.
A J, HODGE, H E, HUXLEY, D, SPIRO
openaire   +2 more sources