Results 251 to 260 of about 27,159 (290)

Milk-clotting activity of cucumisin, a plant serine protease from melon fruit

Applied Biochemistry and Biotechnology, 1996
Cucumisin (EC 3.4.21.25) isolated from prince melon fruit is a plant serine protease. Its milk-clotting activity was compared with plant cysteine proteases such as papain (EC 3.4.22.2) and ficain (EC 3.4.22.3). Cucumisin was more stable than papain under the condition of pH 7.1, 37 degrees C for 24 h.
T, Uchikoba, M, Kaneda
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Production of highly active fungal milk-clotting enzyme by solid-state fermentation

Preparative Biochemistry & Biotechnology, 2019
Cheese production is projected to reach 20 million metric tons by 2020, of which 33% is being produced using calf rennet (EC 3.4.23.4). There is shortage of calf rennet, and use of plant and microbial rennets, hydrolyze milk proteins non-specifically resulting in low curd yields.
Cirium V. Chinmayee, Cheral Vidya, Amsaraj Rani, Sridevi Annapurna Singh   +3 more
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High-Level Expression and Substrate-Binding Region Modification of a Novel BL312 Milk-Clotting Enzyme To Enhance the Ratio of Milk-Clotting Activity to Proteolytic Activity

Journal of Agricultural and Food Chemistry, 2019
A novel BL312 milk-clotting enzyme (MCE) exhibited high-level expression and remarkable milk-clotting activity (MCA) (865 ± 20 SU/mL) that was 3.3-fold higher than the control by optimizing induction conditions in recombinant Escherichia. coli harboring pET24a-proMCE.
Yao Zhang, Yongjun Xia, Xiaofeng Liu, Zhiqiang Xiong, Shijie Wang, Na Zhang, Lianzhong Ai   +6 more
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Proteolytic and milk clotting activities in extracts obtained from the crustaceans Munida

Journal of Molecular Catalysis B: Enzymatic, 2003
Abstract In the present study, individuals of the crustaceans Munida have been investigated as a possible source of enzymes to be used in cheese making as an alternative to calf rennet. The crustaceans were blended and the extracts were filtered and tested for their proteolytic activity and milk clotting capability.
DAMBROSIO A, UNGARO N, ROSSANO, Rocco, RICCIO, Paolo   +3 more
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Site-specific mutations of calf chymosin B which influence milk-clotting activity

Food Chemistry, 1998
Abstract Zymogen forms of wild type and three mutant calf chymosin B enzymes were heterologously expressed in Escherichia coli under control of a T7 promoter as inclusion bodies. The chaperone-like protein, α-crystallin, was used as a possible aid to unfolding.
Supannee Chitpinityol, Derek Goode, M.James C. Crabbe   +2 more
openaire   +1 more source

Expression, activation and processing of a novel plant milk-clotting aspartic protease in Pichia pastoris

Journal of Biotechnology, 2018
Galium verum, also known as Lady's Bedstraw or Cheese Rennet, is an herbaceous perennial plant traditionally used in cheese-making. We used RACE PCR to isolate novel enzymes from Galium verum with the ability to clot milk. This approach generated two cDNA sequences (named preprogaline A and B) encoding proteins displaying the typical plant aspartic ...
Lucía, Feijoo-Siota, José Luis R, Rama, Angeles, Sánchez-Pérez, Tomás G, Villa   +3 more
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Bond specificity, active site and milk clotting mechanism of the Mucor miehei protease

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1972
Mucor miehei produces a protease which is used to replace calf rennet in the cheese industry. Incubation of the enzyme with synthetic substrates indicates that it will hydrolyze peptide bonds having an aromatic amino acid as carboxyl donor. The peptide, carbobenzoxyphenylalanylmethionyl methyl ester containing the bond involved in the milk clotting ...
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Screening of some Egyptian plants for milk clotting activity

Al-Azhar Journal of Agricultural Research, 2022
A. G. Amer, M. A. Omar, N. S. Abdrabou
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Rhizopus and ChlamydomucorStrains Surveyed for Milk-Clotting, Amylolytic, and Antibiotic Activities

Mycologia, 1974
SUMMARY Nine strains of Chlamydomucor oryzae and 347 strains of Rhizopus, representing 10 species, were grown in rice flour and wheat flour media. When grown on wheat flour medium, nearly all strains exhibited amylolytic activity and more than 300 strains showed milk-clotting activity. Almost all strains of R.
John J. Ellis, Hwa L. Wang, Clifford W. Hesseltine   +2 more
openaire   +1 more source

American Cancer Society nutrition and physical activity guideline for cancer survivors

Ca-A Cancer Journal for Clinicians, 2022
Cheryl L Rock, Cynthia A Thomson, Marian L Neuhouser   +2 more
exaly