Results 151 to 160 of about 4,291 (262)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Deed, notes, correspondence, 1877-1931 (Poplar Springs Baptist Church, Zebulon, N.C.)

open access: yes
These documents include a paper slip recording receipts and reimbursements (undated), a request to the church for membership dismission (1877), a portion of a church meeting minutes (undated), and a deed (1882) with a note written in 1931.

core  

Authority of a Notary as Auction Official Class Ii in Implementing Voluntary Non-Execution Auctions at the Request of the Auction House and the Seller at the Office Area of the Directorate General of State Assets Central Java and Diy [PDF]

open access: yes
. Notaries as class II auction officials have the authority to make deeds of minutes auction. This authority still exists among the general public and has not now and understand the authority of a notary as a class II auction official.
Prastyadi, Bahtiar Juniarto   +1 more
core  

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