Results 111 to 120 of about 120,736 (335)
Multi‐Targeting Carnosic Acid Kills Drug‐Resistant Helicobacter pylori With Narrow‐Spectrum Activity
Advanced Science, EarlyView.Carnosic acid selectively eradicates drug‐resistant Helicobacter pylori through a multi‐targeted mechanism involving urease inhibition, membrane disruption, and biofilm eradication. It exhibits no detectable resistance, potent in vivo efficacy, and minimal impact on gut microbiota, positioning it as a promising narrow‐spectrum lead compound against ...
Yuefan Bai +7 more
wiley +1 more source
BMC Bioinformatics, 2020 Background Illumina paired-end reads are often used for 16S analysis in metagenomic studies. Since DNA fragment size is usually smaller than the sum of lengths of paired reads, reads can be merged for downstream analysis.
Yulia Kondratenko +2 more
doaj +1 more source
Primer ID MiSeq Library Prep v4 [PDF]
, 2018 This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire +1 more source
Genes, 2020 The study of DNA to predict externally visible characteristics (EVCs) and the biogeographical ancestry (BGA) from unknown samples is gaining relevance in forensic genetics.
Leire Palencia-Madrid +6 more
semanticscholar +1 more source
Animal Models and Experimental Medicine, EarlyView.Chronic alcohol exposure disrupts intestinal barrier integrity, resulting in a leaky gut and enhanced translocation of lipopolysaccharide (LPS) and microbial products into the systemic circulation. Alcohol‐induced endotoxemia drives exaggerated pro‐inflammatory responses, mitochondrial damage, and activation of the cGAS–STING pathway, particularly in ...
Thansita Bhunyakarnjanarat +5 more
wiley +1 more source
Scientific Reports, 2021 Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding.
Ryohei Nakao +5 more
doaj +1 more source
Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China
OncoTarget, 2017 Pancreatic carcinoma (PC) is a lethal cancer. Gut microbiota is associated with some risk factors of PC, e.g. obesity and types II diabetes. However, the specific gut microbial profile in clinical PC in China has never been reported.
Z. Ren +17 more
semanticscholar +1 more source
A custom software solution for forensic mtDNA analysis of MiSeq data [PDF]
Forensic Science International: Genetics Supplement Series, 2015 Abstract The use of a next generation sequencing (NGS) approach for mitochondrial (mt) DNA analysis in forensic casework is imminent. A feature of the NGS approach is the ability to detect and report mtDNA heteroplasmy, which will significantly enhance the discrimination potential of the testing method.
Mitchell Holland, Jennifer McElhoe
openaire +1 more source
Advanced NanoBiomed Research, EarlyView.CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham +8 more
wiley +1 more source