Results 211 to 220 of about 23,995,846 (308)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Prevalence of stunting and its determinants among children under five in 35 Sub-Saharan African countries (2011-2024): Insights from recent demographic health survey data using a generalized linear mixed-effects model with robust poisson regression. [PDF]

PLoS One
Moloro AH, Sabo KG, Mare KU, Wengoro BF, Endrias EE, Ibrahim RM, Dubie T, Ebrahim OA, Lahole BK.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Long-term metabolic and safety profiles of tenofovir alafenamide and tenofovir disoproxil fumarate in ART-naive people living with HIV: a multicenter retrospective study using a mixed-effects model. [PDF]

BMC Pharmacol Toxicol
Karabay O, Vatan A, Ucar A, Yilmaz I, Balta NC, Kanturk A, Dumlu R, Cag Y, Caskurlu H, Şik MT, Otman ZA, Balci U, Senbayrak S, Akhan S, Deniz MT, Yekenkurul D, Ince N, Tunca B, Cakirca TD, Bastug A, Erkorkmaz U, Guclu E.   +21 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Mixed-Effects Model to Assess the Effect of Disengagements on Speed of an Automated Shuttle with Sensors for Localization, Navigation, and Obstacle Detection. [PDF]

Sensors (Basel)
Grandhi A, Gore N, Pulugurtha SS.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Sleep quality and cognitive functioning among Chinese older adults living in the US: a mixed-effects model analysis. [PDF]

BMC Geriatr
Tang F, Zhu Y, Jayawardena D, Jin G, Jiang Y.   +4 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Did trade unions protect employees' mental health during the COVID-19 pandemic? A mixed effects model using UK data from Understanding Society. [PDF]

BMJ Public Health
Kromydas T, Demou E, Leyland AH, Katikireddi SV, Wels J.   +4 more
europepmc   +1 more source