Results 51 to 60 of about 102,502 (260)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Scaling of Thermal Images at Different Spatial Resolution: The Mixed Pixel Problem

open access: yesAgronomy, 2014
The consequences of changes in spatial resolution for application of thermal imagery in plant phenotyping in the field are discussed. Where image pixels are significantly smaller than the objects of interest (e.g., leaves), accurate estimates of leaf ...
Hamlyn G. Jones, Xavier R. R. Sirault
doaj   +1 more source

Mixed-Pixel Decomposition of SAR Images Based on Single-Pixel ICA with Selective Members

open access: yesGIScience & Remote Sensing, 2011
In the decomposition of synthetic aperture radar (SAR) images with mixed pixels, two issues cannot be avoided; (1) there are more land cover types than the number of SAR image channels; and (2) the...
Yu Xian-chuan   +3 more
openaire   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Unsupervised autoencoder detection with a local context-based correction for mixed pixels in thermal images

open access: yesSmart Agricultural Technology
Thermal imaging is important in areas like precision agriculture, surveillance, and remote sensing because it gives temperature-based information for monitoring environmental conditions. However, mixed pixels often reduce the accuracy of analysis in many
S Sarath   +3 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

A 75-ps Gated CMOS Image Sensor with Low Parasitic Light Sensitivity

open access: yesSensors, 2016
In this study, a 40 × 48 pixel global shutter complementary metal-oxide-semiconductor (CMOS) image sensor with an adjustable shutter time as low as 75 ps was implemented using a 0.5-μm mixed-signal CMOS process.
Fan Zhang, Hanben Niu
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

open access: yesFEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero   +5 more
wiley   +1 more source

SuperBF: Superpixel-Based Bilateral Filtering Algorithm and Its Application in Feature Extraction of Hyperspectral Images

open access: yesIEEE Access, 2019
Bilateral filtering (BF), which is an edge-preserving filtering (EPF) method, has been widely recognized as a simple and efficient approach for hyperspectral image (HSI) feature extraction.
Zhikun Chen   +4 more
doaj   +1 more source

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