Results 61 to 70 of about 100,332 (258)

Spectral unmixing-based Arctic plant species analysis using a spectral library and terrestrial hyperspectral Imagery: A case study in Adventdalen, Svalbard

International Journal of Applied Earth Observations and Geoinformation, 2023
Remote sensing is an invaluable tool for monitoring the rapid changes in Arctic vegetation distribution caused by global warming. Although hyperspectral data consisting of contiguous spectral bands enables the quantitative analysis of remote sensing data,
Junyoung Yang, Yoo Kyung Lee, Junhwa Chi
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A fast Maximum Likelihood method for improving AMCW lidar precision using waveform shape

, 2009
Amplitude Modulated Continuous Wave imaging lidar systems use the time-of-flight principle to determine the range to objects in a scene. Typical systems use modulated illumination of a scene and a modulated sensor or image intensifier.
A. A. Dorrington, Dorrington, Adrian A., Payne, Andrew D., J. P. Godbaz, Godbaz, John Peter, M. J. Cree, A. D. Payne, Cree, Michael J.   +7 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Estimating the number of endmembers in hyperspectral images using the normal compositional model and a hierarchical Bayesian algorithm. [PDF]

, 2010
This paper studies a semi-supervised Bayesian unmixing algorithm for hyperspectral images. This algorithm is based on the normal compositional model recently introduced by Eismann and Stein.
Eches, Olivier, Dobigeon, Nicolas, Tourneret, Jean-Yves   +2 more
core   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

In situ, non-destructive and rapid mineral mapping in tunnels with hyperspectral imaging

Underground Space
Hyperspectral imaging provides a novel approach for intelligent geological perception in tunnelling and underground engineering due to its high spectral resolution, nondestructive nature, and combined spectral-spatial information.
Shan Li, Peng Lin, Kai Yang, Zhenhao Xu
doaj   +1 more source