Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen +11 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Biofabrication aims at providing innovative technologies and tools for the fabrication of tissue‐like constructs for tissue engineering and regenerative medicine applications. By integrating multiple biofabrication technologies, such as 3D (bio) printing with fiber fabrication methods, it would be more realistic to reconstruct native tissue's ...
Waseem Kitana +2 more
wiley +1 more source
Experimental Investigation of Viscous Two-Phase Flow in Microchannels [PDF]
Multi-port microchannel tubes are increasingly popular for use in a variety of heat transfer applications, primarily for automotive condensers and radiators, but also in a variety of refrigeration and air conditioning applications. These channels offer
Burr, J.D., Hrnjak, P.S., Newell, T.A.
core
3D‐Printed Sulfur‐Derived Polymers With Controlled Architectures for Lithium‐Sulfur Batteries
Rheology‐guided formulation design for direct ink writing enables the fabrication of 3D sulfur copolymer cathodes with controlled architectures for lithium‐sulfur batteries. The printed electrodes exhibit multiscale porosity and high sulfur utilization, delivering enhanced electrochemical performance compared to conventional cast electrodes.
Bin Ling +7 more
wiley +1 more source
HVAC SYSTEM REMOTE MONITORING AND DIAGNOSIS OF REFRIGERANT LINE OBSTRUCTION [PDF]
A heating, ventilation, and air conditioning (HVAC) system of a building includes a refrigerant loop. A monitoring system for the HVAC system includes a monitoring device installed at the building.
Alsaleem, Fadi M.
core +1 more source
Development of a dynamic model for ice-on-coil external melt storage systems [PDF]
Ice storage systems are commonly used to balance the intermittency of renewable energy and decrease the peak load by switching to off-peak hours. An adequate model is necessary to predict the behaviour of these systems.
Beyne, Wim +4 more
core +1 more source
Digital Actuation Control of Soft Robotic Origami With Self‐Folding Liquid Crystal Elastomer Hinges
Self‐folding soft‐rigid hybrid robotic origami is enabled by liquid crystal elastomer actuators with embedded Joule heating and closed‐loop digital control. Digitally addressable hinges provide reversible and programmable transitions between distinct folded states while maintaining actuation performance at high cycle counts (over 1500).
David C. Bershadsky +3 more
wiley +1 more source
The Effect of Dissolved Water on the Tribological Properties of Polyalkylene Glycol and Polyolester Oils [PDF]
The effect of water dissolved in polyalkylene glycol and polyolester oils on the tribological behavior of two material contact pairs in three test environments is evaluated.
Cusano, C. +2 more
core
High-global warming potential F-gas emissions in California: comparison of ambient-based versus inventory-based emission estimates, and implications of refined estimates. [PDF]
To provide information for greenhouse gas reduction policies, the California Air Resources Board (CARB) inventories annual emissions of high-global-warming potential (GWP) fluorinated gases, the fastest growing sector of greenhouse gas (GHG) emissions ...
Ashford, Paul +19 more
core +2 more sources

