Results 181 to 190 of about 1,142,402 (245)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Enhancing reproducibility in mixing time determination of stirred tank reactors via automated analysis and standardized inter-laboratory trials. [PDF]

Appl Microbiol Biotechnol
Barth I, Fitschen J, Sieblist C, Husemann U, Kues D, Neubrand C, Bouvier C, Schirmer C, Tscheschke B, Vassilev I, Kampeis P.   +10 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Structure and Catalytic Performance of Aluminum-Based Ozone Catalysts Prepared by a Direct Mixing Method. [PDF]

Chempluschem
Xu C, Zhao X, Chen S, Wan C, Zhou A, Mao X, Duan Y, Wu C.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Spinal cord ischemia in patients on venoarterial extracorporeal membrane oxygenation: a literature review. [PDF]

J Thorac Dis
Worku B, Prario M, Gulkarov I, Gambardella I, Balaram S, Tukacs M, Naka Y, Srivastava A.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Improving the reproducibility of mixing-time experiments in stirred single-use bioreactors. [PDF]

Appl Microbiol Biotechnol
Barth I, Holbeck C, Capitain C, Schirmeister T, Kampeis P.   +4 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

On-chip engineering of EDC-crosslinked antimicrobial peptide hydrogels for enhanced eradication of MRSA infections. [PDF]

RSC Adv
Huang D, Lin F, Yang N, Qian X, Li J, Su M, Hu R, Tian Y, Han F, Guo G.   +9 more
europepmc   +1 more source