Results 41 to 50 of about 3,359,041 (235)

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

open access: yesFEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt   +8 more
wiley   +1 more source

Estimation of tree lists from airborne laser scanning by combining single-tree and area-based methods [PDF]

open access: yes, 2010
Individual tree crown segmentation from airborne laser scanning (ALS) data often fails to detect all trees depending on the forest structure. This paper presents a new method to produce tree lists consistent with unbiased estimates at area level.
Olofsson, Kenneth   +4 more
core   +1 more source

Two-photon laser scanning fluorescence microscopy using photonic crystal fibre [PDF]

open access: yes, 2004
We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques.
McConnell, G., Riis, E.
core   +4 more sources

Analysis of the Qualitative Parameters of Mobile Laser Scanning for the Creation of Cartographic Works and 3D Models for Digital Twins of Urban Areas

open access: yesApplied Sciences
This article focuses on the assessment of point clouds obtained by various laser scanning methods as a tool for 3D mapping and Digital Twin concepts. The presented research employed terrestrial and mobile laser scanning methods to obtain high-precision ...
Ľudovít Kovanič   +4 more
doaj   +1 more source

Retrieval Algorithms for Road Surface Modelling Using Laser-Based Mobile Mapping

open access: yesSensors, 2008
Automated processing of the data provided by a laser-based mobile mapping system will be a necessity due to the huge amount of data produced. In the future, vehiclebased laser scanning, here called mobile mapping, should see considerable use for road ...
Antero Kukko   +3 more
doaj   +1 more source

Mobile 3D scan LiDAR: a literature review

open access: yesGeomatics, Natural Hazards & Risk, 2021
This paper, by critically reviewing different years (from 2010 to 2020) of research activities performed with Mobile Laser Scanning system, aims to review existing systems and how they are exploited in multifaceted domains.
Francesco Di Stefano   +4 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

open access: yesFEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança   +2 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Accuracy Analysis of Convergence Monitoring in Twin-tube Shield Tunnels Using Mobile Laser Scanning

open access: yesChengshi guidao jiaotong yanjiu
[Objective] Mobile 3D laser scanning technology is widely applied in single-tube tunnel monitoring due to its excellent measurement efficiency and high accuracy.
WANG Xinhong   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

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