Results 81 to 90 of about 3,839,063 (319)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Integrating Geovisual Analytics with Machine Learning for Human Mobility Pattern Discovery
ISPRS International Journal of Geo-Information, 2019 Understanding human movement patterns is of fundamental importance in transportation planning and management. We propose to examine complex public transit travel patterns over a large-scale transit network, which is challenging since it involves ...
Tong Zhang +6 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
MobilitApp: Analysing mobility data of citizens in the metropolitan area of Barcelona
, 2016 MobilitApp is a platform designed to provide smart mobility services in urban areas. It is designed to help citizens and transport authorities alike.
Forné, Jordi +4 more
core
A Taxonomy for Management and Optimization of Multiple Resources in Edge Computing
, 2018 Edge computing is promoted to meet increasing performance needs of data-driven services using computational and storage resources close to the end devices, at the edge of the current network.
Nadjm-Tehrani, Simin, Toczé, Klervie
core +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Towards Privacy-Preserving Semantic Mobility Analysis [PDF]
By analyzing data reflecting human mobility, one can derive patterns and knowledge that are tightly linked to the underlying geography and therefore cannot be applied to another territory or even compared with patterns obtained for another territory ...
Andrienko, G., Andrienko, N., Fuchs, G.
core
, 2021 Neste documento é descrito o trabalho realizado no âmbito da disciplina TMDEI do Mestrado em Engenharia de Software (MEI) do Departamento de Engenharia Informática (DEI) do Instituto Superior de Engenharia do Porto (ISEP) do Politécnico do Porto (P.Porto). O trabalho apresentado nesta dissertação foi realizado numa empresa, que por motivos de segurança
openaire +1 more source
FEBS Open Bio, EarlyView.Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source