Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Articulated Untethered Magnetic Actuators for Multimodal and Cross-Scale Operations. [PDF]
Chen Z, Zhao X, Liu Y, Mi S.
europepmc +1 more source
Diffusion-Controlled Nucleation, Growth, and Self-Assembly of Silica Nanoparticles in Laminar Microfluidic Flow. [PDF]
Gomes NMC +5 more
europepmc +1 more source
Nuclear waste radionuclides unveil three decades of anthropogenic carbon in the Canada Basin of the Arctic Ocean. [PDF]
Payne A +7 more
europepmc +1 more source
Direct generation of massive vector-mode entanglement from a polarization-insensitive optical amplifier. [PDF]
Wang X, Wei Y, Zhang K, Pan X, Jing J.
europepmc +1 more source
Assessing Black Carbon and Iron Oxide Aerosols: A Comparative Study between Urban and Rural Environments in the Southeastern U.S. [PDF]
Suri S +9 more
europepmc +1 more source
Impact of mode hybridization on spin-wave profiles in bi-component magnonic crystals. [PDF]
Mamica S.
europepmc +1 more source
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To provide flexibility and efficiency in logic and timing verification of MOS VLSI circuits, it is desirable that various portions of a circuit can be described and simulated at appropriate levels of detail. Such a capability is provided by the Mixed-Mode Simulator described here.
Vishwani D. Agrawal +4 more
openaire +2 more sources

