Purification tags markedly affect self‐aggregation of CPEB3
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito+6 more
wiley +1 more source
Tendency Computations with a Continuous Two-Parametric Atmospheric Model [PDF]
Sigurd J. Smebye
openalex +1 more source
Three-dimensional Model for Cohesive Sediment Transort in Estuary of Palu River [PDF]
Penelitian ini bertujuan untuk mengkaji dinamika transpor sedimen di Estuari Sungai Palu yang memiliki batimetri curam dan perairan yang dangkal di muara.
Lutfi, M. (Mohammad)
core
Circulating histones as clinical biomarkers in critically ill conditions
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez+17 more
wiley +1 more source
Effect of the Model Application Setting Interactive Learning Cooperative Learning with Concern Interest and Capability to Initial Student Learning Outcomes Grade X MIA SMA State 2 Pangkajene [PDF]
Penelitian eksperimen ini bertujuan untuk mengetahui pengaruh model pembelajaran interaktif setting kooperatif dalam pembelajaran matematika siswa kelas X MIA SMA Negeri 2 Pangkajene Tahun Ajaran 2016/2017.
Hapsan, A. (Amran)+1 more
core
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao+7 more
wiley +1 more source
Introducing spectrophotometry in the school lab employing LEGO bricks and LEDs
In this paper we present the simple process of manufacturing a spectrophotometer based on an assembly of LEGO bricks and LED lights. The procedure is simple and the cost is extremely low.
Bouza Myrto-Eleni+3 more
doaj +1 more source
NON-DIMENSIONAL EQUATIONS AND MODELING CRITERIA FOR THE ATMOSPHERE [PDF]
Dave Fultz
openalex +1 more source
Cooperatives a model to improve organic seed production (Liveseed Practice Abstract) [PDF]
Building cooperatives sounds easier than it is. Some general recommendations are: - Build trust – an on-going process - Define clear goals – common aims, clear rules - Have a clear timeline – go step by step - Communicate clearly – among members ...
Nuijten, Edwin
core
Single‐cell insights into the role of T cells in B‐cell malignancies
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley +1 more source