Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Dynamic Prediction of an Event Using Multiple Longitudinal Markers: A Model Averaging Approach. [PDF]
Hashemi R +3 more
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Prognostic Models Using the Lactate-to-Albumin Ratio for 28-Day Mortality in ICU Sepsis Patients: A Bayesian Model Averaging Approach. [PDF]
Hoang QV +4 more
europepmc +1 more source
Robust Bayesian meta-analysis: Model-averaging across complementary publication bias adjustment methods. [PDF]
Bartoš F +4 more
europepmc +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Robust Bayesian model averaging for linear regression models with heavy-tailed errors. [PDF]
De S, Ghosh J.
europepmc +1 more source
Frequentist Model Averaging in Structure Equation Model With Ordinal Data. [PDF]
Jin S.
europepmc +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Overcoming Model Uncertainty - How Equivalence Tests Can Benefit From Model Averaging. [PDF]
Hagemann N, Möllenhoff K.
europepmc +1 more source

