This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
LMRNet: a lightweight convolutional neural network for real-time mountain rice leaf disease recognition on edge devices. [PDF]
Cui J, Yang Y, Chen Z, Hao S, Tan F.
europepmc +1 more source
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Biomechanical Evaluation of Powered Acetabular Extraction Tool Reduces Force, Time, and Surgeon Burden Compared to Manual Tools in a Simulated Revision Total Hip Arthroplasty Model. [PDF]
Mabarak DJ +8 more
europepmc +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Machine learning-driven optimization of extraction process and development of quality standards for traditional Chinese medicine (TCM) formulae in primary liver cancer. [PDF]
Gao X +5 more
europepmc +1 more source
Towards cross-domain few-shot modulation classification: a feature transformation graph neural network approach. [PDF]
Shi Y, Xu H, Qi Z, Wang D, Meng Q.
europepmc +1 more source
Malware detection in IoT networks with CNNs and integrated feature engineering. [PDF]
Abd-Ellah MK +3 more
europepmc +1 more source

