Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
As faces da alteridade e a formação de professores da Licenciatura em Pedagogia da UEPG
O presente trabalho tem por objetivo discutir a noção de alteridade, propondo-se a investigar a forma pela qual esta é constituída no curso de Licenciatura em Pedagogia da Universidade Estadual de Ponta Grossa (UEPG).
Jonathan de O. MOLAR, Priscila LAROCCA
doaj
Molar Incisor Hypomineralization
Enamel defects are common alterations that can occur in both the primary or permanent dentition. A range of etiological factors related to this pathology can be found in the literature. Molar Incisor Hypomineralization(MIH) is a kind of enamel defect alteration that requires complex treatment solutions, and for this reason, it is of great clinical ...
Takahashi, Karine +2 more
openaire +4 more sources
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Ethnic Association of Cusp of Carabelli Trait and Shoveling Trait in an Indian Population [PDF]
Introduction: Variations in the structure of teeth have always been of great interest to the dentist from the scientific as well as practical point of view.
M Kirthiga, M Manju, R Praveen, W Umesh
doaj +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Learning Spreadsheet Tools: The Case of Temperature Dependent Molar Heat Capacity and Change in Molar Enthalpy [PDF]
This paper presents a simple spread sheet tool to calculate a temperature dependent molar heat capacity and change in molar enthalpy from one temperature to another.
Jeremy Lewis
core
The Effect of Second Molar Extraction and Septumotomy on Intraoral Molar Distalization - A Pilot Study [PDF]
This study aimed to evaluate the combined effects of second mo tar extraction and interradicular septum removal on molar distalization, an intraoral distalization mechanism was used, emitting interrupted forces.
Nejat Erverdi +2 more
core +1 more source

