Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Recent advances in π-conjugated organic thermoelectric materials: molecular design, properties, and applications. [PDF]
Rajasekar M.
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Tuning the Twist by Molecular Design: A New Strategy for Hexabenzocoronene-Containing Helical Twistacene. [PDF]
Mora-Fuentes JP +8 more
europepmc +1 more source
Delicate molecular design, self-assembly and functional applications of chiral cyclophanes. [PDF]
Zhang Y, Jia H, Xu L, Liu M, Ouyang G.
europepmc +1 more source
In vivo CAR-T therapy: from molecular design to precision delivery. [PDF]
Li H, Wen W, Zhao X, Liang G.
europepmc +1 more source
Catechol Derivative-Based Bioadhesives: Molecular Design for Precision Medical Adhesion. [PDF]
Wang X +8 more
europepmc +1 more source
Modular Molecular Design and Self-Assembled Nanostructures of Saccharide‑Appended Cyclic Dipeptides for Glycosidase‑Responsive Supramolecular Hydrogels. [PDF]
Sugiura S +6 more
europepmc +1 more source
Toward Real-World Tetracycline Sensing: Label-Free Carbon Quantum Dot Fluorescence From Molecular Design to Matrix-Driven Challenges. [PDF]
Saleh KA +8 more
europepmc +1 more source

