Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Computational evaluation of aluminum and zinc doped C<sub>20</sub> fullerenes as advanced sensors for the detection of the narcotic dimethyltryptamine. [PDF]
Alshahrani SM.
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Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Effect of boron-doping and electric field on CO<sub>2</sub> adsorption on C<sub>60</sub> fullerene. A DFT study. [PDF]
Domínguez-Trujillo MDC +5 more
europepmc +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Unveiling enhanced HCN sensing: a comparative DFT study on reactivity and sensitivity of X-doped biphenylene nanotubes (X = B, Al, and Ga). [PDF]
Hosseini M +4 more
europepmc +1 more source
The latest AI breakthroughs in structural biology: protein binder design and conformational state prediction. [PDF]
Abriata LA.
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One-second-lead earthquake warning and impact assessment at Campi Flegrei. [PDF]
Longobardi V, Colombelli S, Zollo A.
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Future Leaders in Stress: the next generation of stress neurobiology. [PDF]
Bolton JL, Matosin N.
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Research on the influence mechanism of structural separation on the damage excitation of bridge piers under seismic motion. [PDF]
Wen Y, Liu Y, Ning X, Xia H, An W.
europepmc +1 more source

