Results 311 to 320 of about 1,220,527 (364)

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Predicting cerebral palsy and 18-month neurodevelopmental outcome in infants with presumed hypoxic ischaemic encephalopathy: role of general movements assessment and early neurological examination. [PDF]

Front Pediatr
Kali GTJ, du Preez JCF, van Zyl JI, Burger M, Katsabola H, Pepper MS.   +5 more
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Dietary Mannan Oligosaccharides Enhance Lactational Performance, Nutrient Metabolism, Plasma Metabolomics, and Gut Microbiota in Dezhou Donkeys. [PDF]

Int J Mol Sci
Wang T, Wang Y, Li P, Liu J, Mao X, Li Z, Wen Z, Yin Y, Li Y, Lin G, Zhang H, Qu H, Ma Q, Huang S.   +13 more
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Study on the failure mechanism and improvement measures of bearings in the full-ocean-depth ultra-high-pressure seawater pump. [PDF]

Sci Rep
Chen Z, Hu X, Li L, Ye C, Wu D, Liu S, Ma Y, Gu L.   +7 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Fishing out AIEC with FimH capturing microgels for inflammatory bowel disease treatment. [PDF]

Nat Commun
Wu J, Liu Y, Liu R, Xiao C, Xuan L, Wu L, Qian J, Qin X, Hou Y, Xie M, Yu X, Liu B, Tang G.   +12 more
europepmc   +1 more source

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

FEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner, César G. Prucca, Lucia Boffelli, Virginia A. Piqueras, Silvia G. Correa, Mariana Maccioni, Mario E. Guido   +6 more
wiley   +1 more source

Twist tunable spin to charge conversion and valley contrasting effects in graphene on 2D transition metal dichalcogenides. [PDF]

Sci Rep
Wojciechowska I, Dyrdał A.
europepmc   +1 more source