Results 11 to 20 of about 1,006,357 (128)

Methylation Specific PCR (MSP): Nested PCR vs Unnested PCR [PDF]

, 2023
Methylation-specific PCR (MSP) is a valuable technique for studying DNA methylation patterns due to its straightforward design and implementation, high sensitivity in detecting methylated DNA, and ability to analyze large sample sizes cost-effectively ...
Humardani, Farizky Martriano, Tanaya, Lady Theresa Adeodata, Ikawaty, Risma, Putra, Sulistyo Emantoko Dwi, Kesuma, Dini, Wijono, Heru, Sulistomo, Hikmawan Wahyu, Mulyanata, Lisa Thalia   +7 more
core   +1 more source

Biodiagnostics in an era of global pandemics—From biosensing materials to data management

VIEW, Volume 3, Issue 2, March 2022., 2022
This review aims to present a broad overview on the biodiagnostic challenges and potential technological solutions in the era of global pandemics. From the development of new sensors and principals, thorough the establishment of diagnostic approaches and monitoring applications, and ending with management and data analysis challenges.
Yoav Y. Broza, Hossam Haick
wiley   +1 more source

Genetic diversity and phenotypic characterization of Iodobacter limnosediminis associated with skin lesions in freshwater fish

Journal of Fish Diseases, Volume 44, Issue 11, Page 1711-1724, November 2021., 2021
Abstract The relatively unknown genus Iodobacter sp. has been repeatedly isolated from skin ulcers and saprolegniosis on freshwater fish in Finland, especially farmed salmonids. Genetic characterization verified that all 23 bacterial isolates studied here belonged to the species Iodobacter limnosediminis, previously undescribed from the fish microbiota.
Tiina Liisa Korkea‐aho, Satu Viljamaa‐Dirks, Sirpa Heinikainen, Henry Kuronen, Marja Tiirola   +4 more
wiley   +1 more source

Typing of mycoplasma pneumoniae by PCR-mediated DNA fingerprinting [PDF]

, 1994
PCR fingerprinting was used to characterize clinical isolates of Mycoplasma pneumoniae. Among 24 strains tested, two types were distinguished. Nineteen strains belonged to type 1, whereas only 5 strains belonged to type 2.
Ursi, D, Niesters, H.G.M., Quint, W, Ursi, D., Goossens, H, Niesters, H G; id_orcid, van Bever, H, Niesters, H G, Quint, W., Ieven, M, Goossens, H., van Bever, H., Ieven, M., W Quint, H Goossens, H G Niesters, D Ursi, H van Bever, M Ieven   +18 more
core   +5 more sources

Comparative PCR-based fingerprinting of vibrio cholerae isolated in Malaysia [PDF]

, 2011
Vibrio cholerae, the causative agent of cholera, is endemic in many parts of the world, especially in countries poor in resources. Molecular subtyping of V.
Thong, Kwai Lin, Teh, C.S.J., Osawa, R., Cindy Shuan Ju Teh, Ro Osawa, Kwai Lin Thong, Kek Heng Chua, Chue, K.H.   +7 more
core   +2 more sources

Specificity, sensitivity and kappa index of MSP-PCR fingerprinting using the primer (GTG)₅ in comparison with rDNA sequencing as the gold standard for the two datasets (“lower diversity” and “higher diversity”), using two ranges of band sizes: 200–3500bp or 200–900 bp.

, 2014
Specificity, sensitivity and kappa index of MSP-PCR fingerprinting using the primer (GTG)5 in comparison with rDNA sequencing as the gold standard for the two datasets (“lower diversity” and “higher diversity”), using two ranges of band sizes: 200–3500bp
Sandra Denise Camargo Mendes (623310), Patricia Valente (130799), Mauricio Ramírez-Castrillón (623309), Mario Inostroza-Ponta (232809)   +3 more
core   +1 more source

Primer sets used for RT-PCR, Real-time RT-PCR, MSP and pyrosequencing.

, 2014
Abbreviations: HPA: heparanase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MSP: methylation-specific PCR; MSP-U: amplicons for unmethylated MSP; MSP-M: amplicons for methylated MSP; Pyro: pyrosequencing.
Juan Wang (115708), Zhen Yue (535779), Ying Ma (123771), Yuan Yu (241193), Fei Jiao (535776), Shi-yu Bai (535777), Xin Wang (91924), Zhong-hai Yan (535778)   +7 more
core   +1 more source

Dendrograms of the clustering of the strains from the "lower diversity" dataset by Hierarchical Clustering using: (a) average linkage (b) complete linkage, (c) single linkage, and (d) Ward´s method.

, 2014
The distance was computed using the Euclidean distance between the genetic profiles based on the MSP-PCR fingerprinting with the primer GTG5.
Sandra Denise Camargo Mendes (623310), Patricia Valente (130799), Mauricio Ramírez-Castrillón (623309), Mario Inostroza-Ponta (232809)   +3 more
core   +1 more source

Rapid Identification of Edible Insect Species in Food Using MALDI‐TOF Mass Spectrometry

Rapid Communications in Mass Spectrometry, Volume 40, Issue 13, 15 July 2026.
ABSTRACT Rationale Edible insects are emerging as sustainable, nutritious ‘foods of the future’ and are gradually introduced to the European market as novel foods. Ensuring consumer safety and preventing fraud requires legal regulation, which in turn depends on reliable analytical methods.
David Straka, Alena Meledina, Tatiana Anatolievna Smirnova, Jana Hajslova, Lenka Kourimska, Martin Kulma, Katerina Sebelova, Ondrej Pospisil, Anezka Kopecka, Stepanka Kuckova   +9 more
wiley   +1 more source

Acute Inhalation Exposure to Ultrafine Carbon Black Alters Mitochondrial Substrate Utilization in the Heart

Environmental Toxicology, Volume 41, Issue 7, Page 395-406, July 2026.
ABSTRACT A majority of deaths associated with air pollution are attributed to cardiovascular and heart diseases. Carbon black that represents the carbon core of particulate matter, a major component of air pollution, has been shown to result in cardiac mitochondrial dysfunction.
William E. Mullen, Jackson E. Stewart, Rahatul Islam, Salik Hussain, Dharendra Thapa   +4 more
wiley   +1 more source