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Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle [PDF]

Brazilian Journal of Microbiology, 2014
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic ...
CARLOS A N Ramos, Flábio R Araujo, Araujo Flábio R   +2 more
exaly   +5 more sources

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Molecular characterization and phylogenetic analysis of Anaplasma marginale major surface protein (MSP5) gene

Journal of Veterinary Parasitology, 2020
Vikrant Sudan
exaly   +2 more sources

Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein

Research in Veterinary Science, 2014
Anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. Immunization with the current vaccine, based on parasitized erythrocytes with live Anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines.
Nora Mattion
exaly   +4 more sources

Evaluation of a recombinant MSP5 based dot-elisa for serodiagnosis of Anaplasma marginale infection in bovines

Indian Journal of Comparative Microbiology Immunology and Infectious Diseases, 2022
Shobha Yadav, Rajat Garg, Poonam Kumari, Savita Bisen, Hira Ram, O.K. Raina   +5 more
exaly   +2 more sources

ELISA com MSP5 recombinante truncada para detecção de anticorpos contra Anaplasma marginale em bovinos [PDF]

Pesquisa Veterinaria Brasileira, 2007
Os objetivos deste estudo foram produzir e solubilizar a proteína MSP5 recombinante truncada de Anaplasma marginale, e avaliar seu desempenho em um ensaio de imunoadsorção enzimática indireto (ELISA) para detecção de anticorpos contra a riquétsia. O gene msp5, exceto a região N-terminal hidrofóbica, foi amplificado por PCR, clonado em plasmídeo pTrcHis-
Carina Elisei, CARLOS A N Ramos, Cleber O Soares   +2 more
exaly   +4 more sources

Characterization of recombinant MSP5 Anaplasma marginale Havana isolate

Brazilian Journal of Microbiology, 2009
Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5)
Corona, B., Machado, H., Rodriguez, M., Martinez, S.   +3 more
exaly   +8 more sources

Induced immune response of DNA vaccine encoding an association MSP1a, MSP1b, and MSP5 antigens of Anaplasma marginale

Vaccine, 2008
The outer membrane proteins of Anaplasma marginale have been the focus of research to obtain an improved vaccine against bovine anaplasmosis. We evaluated the capacity of the recombinant plasmids pcDNA-msp1alpha, pcDNA-msp1beta, and pcDNA-mp5 to express MSP1a, MSP1b, and MSP5 proteins, and to determine the immunogenicity of BALB/c mice immunized with ...
Odilon Vidotto, Itano E N, João Luis Garcia   +2 more
exaly   +3 more sources

Detection of antibodies against Anaplasma marginale in milk using a recombinant MSP5 indirect ELISA

Veterinary Microbiology, 2005
An indirect enzyme linked immunosorbent assay (iELISA) for diagnosis of anaplasmosis using undiluted individual milk samples from dairy cows was developed. The recombinant 19 kDa major surface protein 5 (rMSP5) of Anaplasma marginale was used as antigen.
Nerina Aguirre
exaly   +3 more sources

Cloning and expression of the gene encoding the major surface protein 5 (MSP5) of Anaplasma phagocytophilum and potential application for serodiagnosis

Veterinary Clinical Pathology, 2006
Abstract Anaplasma phagocytophilum (formerly known as the human granulocytic ehrlichia, Ehrlichia equi and Ehrlichia phagocytophila) is an obligate intracellular organism causing clinical disease in humans and various species of domestic animals. Objectives: The objectives of this investigation were to sequence and clone the major surface protein 5 ...
Alleman, A Rick, Barbet, Anthony F, Sorenson, Heather L, Strik, Nicole I, Wamsley, Heather L, Wong, Susan J, Chandrashaker, Ramaswamy, Gaschen, F, Luckschander, Nicole, Bjöersdorff, Annelli   +9 more
openaire   +3 more sources

Evidencia serológica de Anaplasma spp. en pequeños rumiantes de Venezuela utilizando MSP5 recombinante en ensayos inmunoenzimáticos

2010
Anaplasma marginale ocasiona una enfermedad en los bovinos caracterizada por fiebre, anemia y disminución de la producción de leche y carne. Los pequeños rumiantes generalmente no muestran signos clínicos, por lo que pudieran actuar como reservorio. En Venezuela, los ovinos y caprinos tienen gran importancia económica y socialmente en zonas áridas y ...
Tavares-Marques, Lucinda M, Núñez, Cristina, Rey-Valeirón, Catalina, Reyna-Bello, Armando   +3 more
openaire   +1 more source