Results 11 to 20 of about 72 (65)

Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle.

PLoS ONE, 2019
Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione S-transferase (GST-MSP5). To
María E Primo, Carolina S Thompson, Beatriz S Valentini, Macarena Sarli, María B Novoa, Atilio J Mangold, Susana T de Echaide   +6 more
doaj   +1 more source

Natural infection by Anaplasma marginale during the first weeks of life of calves on a dairy farm in the eastern Amazon [PDF]

Ciência Rural
: This study detected Anaplasma marginale in calvesusing blood smears and nested PCR (nPCR) and to compare the results with the clinical signs presented by calves on a dairy farm in the municipality of Castanhal, located northeast of the state Pará (1°07’
Maiara Vasconcelos Monteiro, Danillo Henrique da Silva Lima, Matheus Dias Cordeiro, Bruna de Azevedo Baêta, Izabela Mesquita Araújo, Maria Hilma Soares Sodré, Mário José Costa Carneiro, Nailson de Andrade Neri Junior, Ediene Moura Jorge, Paulo Cesar Magalhães-Matos, José Diomedes Barbosa Neto, Natália da Silva e Silva Silveira   +11 more
doaj   +1 more source

Direct PCR-RFLP based detection and differentiation of Anaplasma species in naturally infected goats of eastern Haryana, India

Indian Journal of Animal Sciences, 2019
The present investigation was designed to develop a novel, rapid and cost effective direct PCR-RFLP assay, as a specific diagnostic tool for detection and differentiation of two Anaplasma species (A. ovis and A. marginale).
BISWA RANJAN MAHARANA, ANITA GANGULY, RANBIR SINGH BISLA, DEVAN ARORA, ANKIT KUMAR, SANDEEP POTLIYA, CHINMOY MISHRA, INDRAJIT GANGULY   +7 more
doaj   +1 more source

Molecular detection of tick‐borne pathogens in bovine blood and ticks from Khentii, Mongolia

Transboundary and Emerging Diseases, Volume 67, Issue S2, Page 111-118, July 2020., 2020
Abstract Recent studies reported the detection of DNA from tick‐borne pathogens (TBPs) of veterinary relevance such as Anaplasma marginale, Babesia bigemina, Babesia bovis and Theileria orientalis in bovine blood samples from Mongolia. These findings were unexpected, as the known tick vectors of these pathogens are not known to occur in Mongolia.
Timon Fischer, Mungunbariya Myalkhaa, Jürgen Krücken, Gonchigoo Battsetseg, Zayat Batsukh, Maximilian P. O. Baumann, Peter‐Henning Clausen, Ard M. Nijhof   +7 more
wiley   +1 more source

Molecular diagnosis of Anaplasma marginale in cattle: quantitative evaluation of a real-time PCR (Polymerase Chain Reaction) based on msp5 gene

Pesquisa Veterinária Brasileira, 2014
The rickettsia Anaplasma marginale is considered the main agent of bovine anaplasmosis. Due the nonspecific clinical signs of the anaplasmosis, the diagnosis of infection depends of laboratory confirmation.
Gisele M. Bacanelli, Carlos A. N. Ramos, Flábio R. Araújo   +2 more
doaj   +1 more source

Transmission of Anaplasma marginale by unfed Rhipicephalus microplus tick larvae under experimental conditions

Revista Mexicana de Ciencias Pecuarias, 2020
The current description of biological transmission of Anaplasma marginale by Rhipicephalus microplus ticks, includes of the biological intrastadial and transstadial transmission.
Itzel Amaro Estrada, Miguel A. García-Ortiz, Jesús F. Preciado de la Torre, Edmundo E. Rojas-Ramírez, Rubén Hernández-Ortiz, Francisco Alpírez-Mendoza, Sergio D. Rodríguez-Camarillo   +6 more
doaj   +1 more source

Immunoinformatic Analysis to Identify Proteins to Be Used as Potential Targets to Control Bovine Anaplasmosis

International Journal of Microbiology, Volume 2020, Issue 1, 2020., 2020
Omics sciences and new technologies to sequence full genomes provide valuable data that are revealed only after detailed bioinformatic analysis is performed. In this work, we analyzed the genomes of seven Mexican Anaplasma marginale strains and the data from a transcriptome analysis of the tick Rhipicephalus microplus.
Sergio D. Rodríguez-Camarillo, Rosa E. Quiroz-Castañeda, Hugo Aguilar-Díaz, José E. Vara-Pastrana, Diego Pescador-Pérez, Itzel Amaro-Estrada, Fernando Martínez-Ocampo, Todd R. Callaway   +7 more
wiley   +1 more source

Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau

Animals, 2022
Anaplasma genus infects the blood cells of humans and animals by biting, causing zoonotic anaplasmosis. However, limited data are available on carrier animals for Anaplasma spp. antibodies in the Qinghai–Tibetan Plateau Area.
Jinchao Zhang, Hejia Ma, Jingkai Ai, Tongsheng Qi, Ming Kang, Jixu Li, Yali Sun   +6 more
doaj   +1 more source

Seasonal Investigation of Anaplasma marginale Infection in Pakistani Cattle Reveals Hematological and Biochemical Changes, Multiple Associated Risk Factors and msp5 Gene Conservation

Pathogens, 2022
Bovine anaplasmosis is a tick-borne disease caused by an obligate intercellular Gram-negative bacterium named Anaplasma (A.) marginale. In this study, we report the seasonal prevalence, potentially associated risk factors and phylogeny of A. marginale in
Muhammad Asif, Mourad Ben Said, Rommel Lenin Vinueza, Renato Leon, Nadeem Ahmad, Asia Parveen, Adil Khan, Arusa Ejaz, Muhammad Ali, Asmat Ullah Khan, Muhammad Baber, Furhan Iqbal   +11 more
doaj   +1 more source

AFCo1, a meningococcal B-derived cochleate adjuvant, strongly enhances antibody and T-cell immunity against Plasmodium falciparum merozoite surface protein 4 and 5

Malaria Journal, 2009
Background Whilst a large number of malaria antigens are being tested as candidate malaria vaccines, a major barrier to the development of an effective vaccine is the lack of a suitable human adjuvant capable of inducing a strong and long lasting immune ...
Pérez Oliver, Coppel Ross, Wang Lina, Zayas Caridad, Bracho Gustavo, Petrovsky Nikolai   +5 more
doaj   +1 more source