Results 11 to 20 of about 559 (129)

Molecular diagnosis of Anaplasma marginale in cattle: quantitative evaluation of a real-time PCR (Polymerase Chain Reaction) based on msp5 gene [PDF]

Pesquisa Veterinaria Brasileira, 2014
The rickettsia Anaplasma marginale is considered the main agent of bovine anaplasmosis. Due the nonspecific clinical signs of the anaplasmosis, the diagnosis of infection depends of laboratory confirmation.
CARLOS A N Ramos, Flábio R Araujo, Araujo Flábio R   +2 more
exaly   +5 more sources

Soroprevalência de Anaplasma marginale em bovinos da região Centro-Sul do estado do Paraná, Brasil, por um teste imunoenzimático competitivo utilizando proteína recombinante MSP5-PR1 Seroprevalence of Anaplasma marginale in cattle from Center-South Region of Paraná State, Brazil by a competitive ELISA test with recombinant MSP5-PR1 protein

Revista Brasileira de Parasitologia Veterinária, 2009
Foi determinada a prevalência de Anaplasma marginale em 223 soros de bovinos com idade maior ou igual a dois anos, das regiões de Ponta Grossa, Guarapuava e Laranjeiras do Sul, região Centro-Sul do estado do Paraná.
Elizabete Regina Marangoni Marana, Juliana Alves Dias, Roberta Lemos Freire, Josy Campanhã Vicentini, Marilda Carlos Vidotto, Odilon Vidotto   +5 more
doaj   +7 more sources

ELISA com MSP5 recombinante truncada para detecção de anticorpos contra Anaplasma marginale em bovinos ELISA based on recombinant truncated MSP5 for detection of antibodies against Anaplasma marginale in cattle

Pesquisa Veterinária Brasileira, 2007
Os objetivos deste estudo foram produzir e solubilizar a proteína MSP5 recombinante truncada de Anaplasma marginale, e avaliar seu desempenho em um ensaio de imunoadsorção enzimática indireto (ELISA) para detecção de anticorpos contra a riquétsia. O gene
Elaine S.P. Melo, Flábio R. Araújo, Carlos A.N. Ramos, Cleber O. Soares, Grácia M.S. Rosinha, Carina Elisei, Cláudio R. Madruga   +6 more
doaj   +2 more sources

CRISPR-Cas-Based Pen-Side Diagnostic Tests for Anaplasma marginale and Babesia bigemina [PDF]

Microorganisms
Anaplasma marginale and Babesia bigemina are tick-borne pathogens, posing significant threats to the health and productivity of cattle in tropical and subtropical regions worldwide.
Robert Muriuki, Maingi Ndichu, Samuel Githigia, Nicholas Svitek   +3 more
doaj   +2 more sources

Molecular detection and characterization of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in sheep and goats in Luxor, Egypt [PDF]

BMC Veterinary Research
Background Tick-borne diseases cause economically significant losses to animal production globally, and anaplasmosis and theileriosis are associated with the greatest losses. However, the spread of the relevant pathogens in flocks of domesticated animals
Hassan Y. A. H. Mahmoud, Tetsuya Tanaka, Alsagher O. Ali, Walaa F. A. Emeish   +3 more
doaj   +2 more sources

Molecular Identification of Babesia spp. and Anaplasma marginale in Water Buffaloes in Veracruz and Tabasco, Mexico: A Retrospective Study [PDF]

Microorganisms, 2022
Two hundred and thirty-three blood samples of water buffalo were collected on four farms in Veracruz state and Tabasco state, Mexico, to detect and confirm the identities of Babesia and Anaplasma spp. sequences.
José Juan Lira-Amaya, Rebeca Montserrat Santamaria-Espinosa, Roberto O. Castañeda-Arriola, Grecia Martínez-García, Diego J. Polanco-Martínez, Carmen Rojas-Martínez, Jesús Ántonio Alvarez-Martínez, Julio V. Figueroa-Millán   +7 more
doaj   +2 more sources

Genetic diversity of merozoite surface protein-5 (MSP-5) of Plasmodium vivax isolates from Malaria patients in Iran [PDF]

BMC Infectious Diseases, 2023
Malaria has not yet been eradicated in Iran, and Plasmodium vivax (P. vivax) is the main cause of malaria in the country. This study aimed to investigate and analyze the amount of genetic diversity of Plasmodium vivax merozoite surface protein-5 (PvMSP-5)
Sholeh Mansouri, Aliehsan Heidari, Hossein Keshavarz, Parviz Fallah, Amir Bairami, Elaheh Mahmoudi   +5 more
doaj   +2 more sources

Serological evidence of anaplasma spp. in small ruminants from Venezuela using recombinant msp5 in immunoenzymatic assay

Revista Científica, 2010
Anaplasma marginale causes a disease in cattle characterized by fever, anemia and decrease in milk and meat production. Small ruminants do not show signs of disease when infected, but it has been suggested they could act as reservoirs.
Lucinda Tavares Marques, Cristina Núñez, Catalina Rey Valeirón, Armando Reyna Bello   +3 more
doaj   +1 more source

Clonagem, expressão, caracterização molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicação em um teste de ELISA por competição Cloning, expression, molecular characterization of the MSP5 protein from PR1 strain of Anaplasma marginale and its application in a competitive enzyme-linked immunosorbent test

Revista Brasileira de Parasitologia Veterinária, 2009
No presente trabalho, um teste imunoenzimático por competição (cELISA) foi padronizado com a proteína recombinante de superfície rMSP5, clonada a partir do gene msp5 do isolado PR1 de A. marginale. O sequenciamento mostrou que o gene que codifica a rMSP5/
Elizabete Regina M. Marana, Flora S. Kano, Josy C. Vicentini, Rafael S. Spurio, Marcela Ribeiro, Adriana Letícia M. Coelho, Marilda C. Vidotto, Odilon Vidotto   +7 more
doaj   +4 more sources

Loop-mediated isothermal amplification assay for the rapid detection of Anaplasma marginale in cattle based on major surface protein 5 gene

Journal of Vector Borne Diseases, 2023
Background & objectives: Timely intervention is needed to minimize the economic losses of vector-borne bovine anaplasmosis which can be possible by the isothermal amplification assay. Methods: Anaplasma marginale in the cattle of south Gujarat, India was
Nabanita Ganguly, Niranjan Kumar, Jayesh B Solanki, I H Kalyani, Nabanita Thakuria, Dharmesh C Patel   +5 more
doaj   +1 more source