Results 31 to 40 of about 849 (155)

Efficient and selective isotopic labeling of hemes to facilitate the study of multiheme proteins

BioTechniques, 2012
Specific isotopic labeling of hemes provides a unique opportunity to characterize the structure and function of heme-proteins. Unfortunately, current methods do not allow efficient labeling in high yields of multiheme cytochromes c, which are of great ...
Bruno M. Fonseca, Ming Tien, Mario Rivera, Liang Shi, Ricardo O. Louro   +4 more
doaj   +1 more source

Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.

PLoS Biology, 2022
The anaerobic oxidation of methane coupled to sulfate reduction is a microbially mediated process requiring a syntrophic partnership between anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB).
Grayson L Chadwick, Connor T Skennerton, Rafael Laso-Pérez, Andy O Leu, Daan R Speth, Hang Yu, Connor Morgan-Lang, Roland Hatzenpichler, Danielle Goudeau, Rex Malmstrom, William J Brazelton, Tanja Woyke, Steven J Hallam, Gene W Tyson, Gunter Wegener, Antje Boetius, Victoria J Orphan   +16 more
doaj   +1 more source

Multiheme Cytochromes from the Sulfur‐Reducing Bacterium Desulfuromonas Acetoxidans [PDF]

European Journal of Biochemistry, 1997
Two new multiheme cytochromes were isolated from the anaerobic sulfur reducing bacterium Desulfuromonas acetoxidans. They have monomeric molecular masses of 50 and 65 kDa and contain six and eight hemes, respectively. Visible and EPR spectroscopies, in the as‐isolated (oxidised) cytochromes, show the presence of only low‐spin hemes in the 50‐kDa ...
I A, Pereira, I, Pacheco, M Y, Liu, J, Legall, A V, Xavier, M, Teixeira   +5 more
openaire   +2 more sources

Comparative structure-potentio-spectroscopy of the Shewanella outer membrane multiheme cytochromes [PDF]

Current Opinion in Electrochemistry, 2017
Summary Many species of bacteria can generate energy in the anoxic subsurface by directly coupling intracellular oxidative reactions to the reduction of extracellular metal oxides. Coupling these processes requires electron transfer networks that extend from the inside of the cell, across the outer membrane to the extracellular terminal electron ...
Edwards, Marcus J., Gates, Andrew J., Butt, Julea N., Richardson, David J., Clarke, Thomas A.   +4 more
openaire   +2 more sources

Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens: Biochemical Characterization of Cytochrome CbcL

Frontiers in Microbiology, 2022
Exoelectrogenic microorganisms are in the spotlight due to their unique respiratory mechanisms and potential applications in distinct biotechnological fields, including bioremediation, bioenergy production and microbial electrosynthesis.
Jorge M. A. Antunes, Jorge M. A. Antunes, Marta A. Silva, Marta A. Silva, Carlos A. Salgueiro, Carlos A. Salgueiro, Leonor Morgado, Leonor Morgado   +7 more
doaj   +1 more source

Response of the Anaerobic Methanotrophic Archaeon Candidatus “Methanoperedens nitroreducens” to the Long-Term Ferrihydrite Amendment

Frontiers in Microbiology, 2022
Anaerobic methanotrophic (ANME) archaea can drive anaerobic oxidation of methane (AOM) using solid iron or manganese oxides as the electron acceptors, hypothetically via direct extracellular electron transfer (EET).
Chen Cai, Chen Cai, Gaofeng Ni, Jun Xia, Xueqin Zhang, Yue Zheng, Bingqing He, Bingqing He, Esteban Marcellin, Weiwei Li, Weiwei Li, Jiaoyang Pu, Zhiguo Yuan, Shihu Hu   +13 more
doaj   +1 more source

How Thermophilic Gram-Positive Organisms Perform Extracellular Electron Transfer: Characterization of the Cell Surface Terminal Reductase OcwA

mBio, 2019
Extracellular electron transfer is the key process underpinning the development of bioelectrochemical systems for the production of energy or added-value compounds.
N. L. Costa, B. Hermann, V. Fourmond, M. M. Faustino, M. Teixeira, O. Einsle, C. M. Paquete, R. O. Louro   +7 more
doaj   +1 more source

Discovery of a functional, contracted heme-binding motif within a multiheme cytochrome [PDF]

Journal of Biological Chemistry, 2019
Anaerobic ammonium-oxidizing (anammox) bacteria convert nitrite and ammonium via nitric oxide (NO) and hydrazine into dinitrogen gas by using a diverse array of proteins, including numerous c-type cytochromes. Many new catalytic and spectroscopic properties of c-type cytochromes have been unraveled by studies on the biochemical pathways underlying the ...
Ferousi, Christina, Lindhoud, Simon, Baymann, Frauke, Hester, Eric R., Reimann, Joachim, Kartal, Boran   +5 more
openaire   +6 more sources

Anodic and Cathodic Extracellular Electron Transfer by the Filamentous Bacterium Ardenticatena maritima 110S

Frontiers in Microbiology, 2018
Ardenticatena maritima strain 110S is a filamentous bacterium isolated from an iron-rich coastal hydrothermal field, and it is a unique isolate capable of dissimilatory iron or nitrate reduction among the members of the bacterial phylum Chloroflexi. Here,
Satoshi Kawaichi, Tetsuya Yamada, Tetsuya Yamada, Akio Umezawa, Shawn E. McGlynn, Shawn E. McGlynn, Takehiro Suzuki, Naoshi Dohmae, Takashi Yoshida, Yoshihiko Sako, Nobuhiro Matsushita, Kazuhito Hashimoto, Ryuhei Nakamura, Ryuhei Nakamura   +13 more
doaj   +1 more source

Characterization of the periplasmic redox network that sustains the versatile anaerobic metabolism of Shewanella oneidensis MR-1

Frontiers in Microbiology, 2015
The versatile anaerobic metabolism of the Gram-negative bacterium Shewanella oneidensis MR-1 (SOMR-1) relies on a multitude of redox proteins found in its periplasm.
Mónica N. Alves, Sónia E. Neto, Alexandra S. Alves, Afonso eCarrêlo, Isabel ePacheco, Bruno M. Fonseca, Catarina M. Paquete, Cláudio M. Soares, Ricardo O Louro   +8 more
doaj   +1 more source