Results 241 to 250 of about 20,794,413 (306)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

UAV Path Optimization for Target Passive Localization Considering the Position Uncertainty of the Target. [PDF]

Sensors (Basel)
Lin J, Song L, Lu Y, Li X, Li W, Xu G.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Real-world Roche Elecsys^® and Fujirebio Lumipulse^® inter-platform comparability and clinical validation of plasma neurofilament light chain in multiple sclerosis. [PDF]

J Neurol
Criscuolo V, Nicolella V, Polito C, Sirica R, Fiorenza M, Sorvillo D, Cave ID, Carotenuto A, Petracca M, Lanzillo R, Castaldo G, Morra VB, Terracciano D, Moccia M.   +13 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Medical mistrust, health system distrust, and their associations with healthcare delay and cancer screening attitudes in primary care. [PDF]

BMC Health Serv Res
Uçar M, Yi̇ği̇t MF, Kaplan M.
europepmc   +1 more source

Lorenz-Gini method for quantitative assessment of particle distribution in composite microstructures from SEM feature areas. [PDF]

MethodsX
Ben F.
europepmc   +1 more source

Improving the Quality of Lyophilized Animal Vaccines: A Review of Optimization Strategies for Freeze-Drying Processes. [PDF]

Vet Sci
Huang F, Jiao J, Wang Z, Jie H, He J, Zuo X, Zhao Y, Lu Y, Gu J, Lyu F.   +9 more
europepmc   +1 more source

Teachers' Responses to Cyberbullying: Psychometric Validation and Latent Profile Analysis Among Chinese Teachers. [PDF]

Behav Sci (Basel)
Sun M, Lu Z, Luo J.
europepmc   +1 more source

High-Fidelity Simulation for Medical Students: Does Psychological Safety Increase During the Prebriefing Phase? [PDF]

Cureus
Hodge TJ, Kooken JW.
europepmc   +1 more source