Results 181 to 190 of about 669,844 (267)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Multitrait Evaluation of Drought Tolerance at the Seedling Stage in Advanced Backcross Germplasm Derived from Sugarcane × <i>Tripidium arundinaceum</i>. [PDF]

Plants (Basel)
Liu J, Zhang Y, Ye Y, Yu B, Cheng C, Liao Y, Huang C, Wu J, Zhao X, Deng Z.   +9 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Broad environmental adaptation is associated with root anatomical phenotypes in maize landraces: an in silico study. [PDF]

Ann Bot
Lopez-Valdivia I, Rangarajan H, Vallebueno-Estrada M, Lynch JP.   +3 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Overexpression of <i>ROSMARINIC ACID SYNTHASE</i> enhances the hepatoprotective potential of <i>Dracocephalum heterophyllum</i> hairy root cultures. [PDF]

aBIOTECH
Cao L, Wu Z, Cao Y, Ren L, Hu N, Liu Y, Liu Y, Yu S, Sun J, Liu M, Yuan F, Wang H, Fu C.   +12 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A STRUBBELIG-NHL3 cell surface receptor complex mediates the response to cellulose deficiency in Arabidopsis. [PDF]

Nat Plants
Boikine R, Chaudhary A, Mergner J, Leicher H, Stegmann M, Kuster B, Schneitz K.   +6 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

The Melatonin-Nitric Oxide Signaling Pathway Alleviates Iron Starvation by Mobilizing Apoplastic Iron Pools and Reshaping Iron Transport in Rice (Oryza sativa L.). [PDF]

J Pineal Res
Huang J, Wang HY, Jing ZT, Xu YG, Wang SW, Zhang G, Shen RF, Zheng L, Liu TW, Zhu XF.   +9 more
europepmc   +1 more source