Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Seeing the big picture: imaging in immunotherapy. [PDF]
Lau D.
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Machine Learning-Based Fatigue Detection from EEG Signals: A Methodological Pilot Framework with Potential for Nursing Applications. [PDF]
Cetin S, Dagdevir E.
europepmc +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Psychometric properties of a short form for the Coping Inventory for Stressful Situations (CISS-SF). [PDF]
Van Elswyk A +3 more
europepmc +1 more source
First Observation of Wild Double-Flowered <i>Rhododendron nivale</i> With Stamen Petaloidy and Possible Parallel Evolution. [PDF]
Chen X, Deng S, Wang Z.
europepmc +1 more source
Single and Multiple Infestation by Coccoid Pests in Coffee Shape Plant Chemical Defenses and Interactions Across Trophic Levels. [PDF]
Carmo EBS +8 more
europepmc +1 more source
Remote Sensing for Operational Surveillance of Mosquito-Borne Diseases. [PDF]
Ash LV +8 more
europepmc +1 more source
Emergent multiscale organisation of neural dynamics fragments in anaesthesia. [PDF]
Milinkovic B +4 more
europepmc +1 more source

