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Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per
W. Tan +8 more
semanticscholar +1 more source
Signaling circuits often coordinate cellular membranes and actin filaments at distinct sites to direct cell behavior. In this issue of Developmental Cell, Bershteyn et al. outline how the molecular scaffold protein, MIM, which bends membranes and binds actin filaments, is at the middle of one such circuit to regulate ciliogenesis.
Kozminski, Keith G., Schafer, Dorothy A.
openaire +2 more sources
Given a classical algebraic structure—e.g. a monoid or group—with carrier set X, and given a positive integer n, there is a canonical way of obtaining the same structure on carrier set Xn by defining the required operations “pointwise”. For resource-sensitive algebra (i.e.
Chantawibul, Apiwat, Sobocinski, Pawel
openaire +2 more sources
Multiplex Pyrosequencing [PDF]
We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation and hybridization of multiple sequencing primers ...
Nader, Pourmand +3 more
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Completing bacterial genome assemblies with multiplex MinION sequencing
Illumina sequencing platforms have enabled widespread bacterial whole genome sequencing. While Illumina data is appropriate for many analyses, its short read length limits its ability to resolve genomic structure. This has major implications for tracking
R. Wick, L. Judd, C. Gorrie, K. Holt
semanticscholar +1 more source
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019, and the outbreak rapidly evolved into the current coronavirus disease pandemic. SARS-CoV-2 is a respiratory virus that causes symptoms similar to those caused by influenza
Bo Shu +21 more
doaj +1 more source
Integrated textile sensor patch for real-time and multiplex sweat analysis
We report a sweat analysis patch based on silk-derived carbon textile for simultaneous detection of six health-related biomarkers. Wearable sweat analysis devices for monitoring of multiple health-related biomarkers with high sensitivity are highly ...
Wenya He +8 more
semanticscholar +1 more source
Background and aim Plasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable colistin resistance genes (mcr-1 ...
A. R. Rebelo +19 more
semanticscholar +1 more source
Coevolution and Correlated Multiplexity in Multiplex Networks [PDF]
5 pages, 4 figures, published in ...
Kim, Jung Yeol, Goh, K. -I.
openaire +3 more sources

