Results 31 to 40 of about 558,709 (274)

MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection

Pathogens, 2023
A multiplexed enzyme-linked immunosorbent assay (ELISA) that simultaneously measures antibody binding to multiple antigens can extend the impact of serosurveillance studies, particularly if the assay approaches the simplicity, robustness, and accuracy of
Janie R. Byrum, Eric Waltari, Owen Janson, Syuan-Ming Guo, Jenny Folkesson, Bryant B. Chhun, Joanna Vinden, Ivan E. Ivanov, Marcus L. Forst, Hongquan Li, Adam G. Larson, Lena Blackmon, Ziwen Liu, Wesley Wu, Vida Ahyong, Cristina M. Tato, Krista M. McCutcheon, Rebecca Hoh, J. Daniel Kelly, Jeffrey N. Martin, Michael J. Peluso, Timothy J. Henrich, Steven G. Deeks, Manu Prakash, Bryan Greenhouse, Shalin B. Mehta, John E. Pak   +26 more
doaj   +1 more source

Development of a multiplex PCR for the genetic analysis of paddlefish (Polyodon spathula Walbaum,1792) populations

Ribogospodarsʹka Nauka Ukraïni, 2017
Purpose. Paddlefish is commercially important species owing to its biological features and consumer characteristics, namely it produces valuable and delicious fish products, such as high quality meat and black caviar.
K. Kurta, O. Malysheva, V. Spyrydonov
doaj   +1 more source

Systematic evaluation of SARS‐CoV‐2 antigens enables a highly specific and sensitive multiplex serological COVID‐19 assay

Clinical & Translational Immunology, 2021
Objective The COVID‐19 pandemic poses an immense need for accurate, sensitive and high‐throughput clinical tests, and serological assays are needed for both overarching epidemiological studies and evaluating vaccines. Here, we present the development and
Sophia Hober, Cecilia Hellström, Jennie Olofsson, Eni Andersson, Sofia Bergström, August Jernbom Falk, Shaghayegh Bayati, Sara Mravinacova, Ronald Sjöberg, Jamil Yousef, Lovisa Skoglund, Sara Kanje, Anna Berling, Anne‐Sophie Svensson, Gabriella Jensen, Henric Enstedt, Delaram Afshari, Lan Lan Xu, Martin Zwahlen, Kalle vonFeilitzen, Leo Hanke, Ben Murrell, Gerald McInerney, Gunilla B Karlsson Hedestam, Christofer Lendel, Robert G Roth, Ingmar Skoog, Elisabet Svenungsson, Tomas Olsson, Anna Fogdell‐Hahn, Ylva Lindroth, Maria Lundgren, Kimia T Maleki, Nina Lagerqvist, Jonas Klingström, Rui Da Silva Rodrigues, Sandra Muschiol, Gordana Bogdanovic, Laila Sara Arroyo Mühr, Carina Eklund, Camilla Lagheden, Joakim Dillner, Åsa Sivertsson, Sebastian Havervall, Charlotte Thålin, Hanna Tegel, Elisa Pin, Anna Månberg, My Hedhammar, Peter Nilsson   +49 more
doaj   +1 more source

Development of a Multiplex Polymerase Chain Reaction-Based DNA Lateral Flow Assay as a Point-of-Care Diagnostic for Fast and Simultaneous Detection of MRSA and Vancomycin Resistance in Bacteremia

Diagnostics, 2022
To reduce high mortality and morbidity rates, timely and proper treatment of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infection is required.
Mona T. Kashef, Omneya M. Helmy
doaj   +1 more source

A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival

Molecular Oncology, EarlyView.
There is an unmet need in metastatic breast cancer patients to monitor therapy response in real time. In this study, we show how a noninvasive and affordable strategy based on sequencing of plasma samples with longitudinal tracking of tumour fraction paired with a statistical model provides valuable information on treatment response in advance of the ...
Emma J. Beddowes, Mario Ortega Duran, Solon Karapanagiotis, Alistair Martin, Meiling Gao, Riccardo Masina, Ramona Woitek, James Tanner, Fleur Tippin, Justine Kane, Jonathan Lay, Anja Brouwer, Stephen‐John Sammut, Suet‐Feung Chin, Davina Gale, Dana W. Y. Tsui, Sarah‐Jane Dawson, Nitzan Rosenfeld, Maurizio Callari, Oscar M. Rueda, Carlos Caldas   +20 more
wiley   +1 more source

Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6

eLife, 2017
Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system.
Rasmus O Bak, Daniel P Dever, Andreas Reinisch, David Cruz Hernandez, Ravindra Majeti, Matthew H Porteus   +5 more
doaj   +1 more source

Assessing respiratory viral exclusion and affinity interactions through co-infection incidence in a pediatric population during the 2022 resurgence of influenza and RSV

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionIn the Northeast US, respiratory viruses such as influenza and respiratory syncytial virus (RSV), which were largely suppressed by COVID-19-related social distancing, made an unprecedented resurgence during 2022, leading to a substantial rise
Maxwell D. Weidmann, Daniel A. Green, Gregory J. Berry, Fann Wu   +3 more
doaj   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

A Belgian Serosurveillance/Seroprevalence Study of Diphtheria, Tetanus and Pertussis Using a Luminex xMAP Technology-Based Pentaplex

Vaccines, 2016
Serosurveillance and seroprevalence studies are an essential tool to monitor vaccine-preventable diseases. We have developed a magnetic bead-based pentaplex immunoassay (MIA) for the simultaneous detection of IgG antibodies against diphtheria toxin (DT),
Raissa Nadège Caboré, Denis Piérard, Kris Huygen   +2 more
doaj   +1 more source

Modeling the Multi-layer Nature of the European Air Transport Network: Resilience and Passengers Re-scheduling under random failures

, 2012
We study the dynamics of the European Air Transport Network by using a multiplex network formalism. We will consider the set of flights of each airline as an interdependent network and we analyze the resilience of the system against random flight ...
Boccaletti, Stefano, Cardillo, Alessio, del Amo, Alejandro J. García, Gómez-Gardeñes, Jesús, Romance, Miguel, Zanin, Massimiliano   +5 more
core   +1 more source