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Diagnosing superinfection keratitis with multiplex polymerase chain reaction
Journal of Infection and Chemotherapy, 2018To report the potential usefulness of multiplex polymerase chain reaction (mPCR) for diagnosing superinfection keratitis caused by herpes simplex virus-1 (HSV-1), bacteria and fungus.Case series. Corneal scrapings were analyzed with mPCR for human herpes virus 1-8, bacterial 16S ribosomal DNA (rDNA) and fungal 28S rDNA.Case 1 was a 69-year-old man who ...
Masaaki, Yoshida +8 more
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Multiplex gene removal by two-step polymerase chain reactions
Analytical Biochemistry, 2015Precise DNA manipulation is critical for molecular biotechnology. Restriction enzyme-based approaches are limited by their requirement of specific enzyme sites. Restriction-free cloning has greatly improved the flexibility and speed of precise DNA assembly. Most of these approaches focus on DNA assembly rather than gene removal.
Vishnu Vardhan, Krishnamurthy +4 more
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Human sex determination using multiplex polymerase chain reaction (PCR)
Science & Justice, 1996A sex determination method has been developed using the polymerase chain reaction (PCR) which involved the amplification of the sex-determining region Y (SRY) gene and the amplification of the HLA-DQa gene as an internal control. This method, which can be applied to old and degraded DNA samples, allowed confident sexing of biological samples producing ...
Finch, Jennie Louise +2 more
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Multiplexed digital polymerase chain reaction as a powerful diagnostic tool
Biosensors and Bioelectronics, 2021The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced.
Martina Gaňová +4 more
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Multiplex polymerase chain reaction of Alu polymorphic insertions
ELECTROPHORESIS, 1998AbstractAlu sequences are present in humans in excess of 500 000 copies per haploid genome and represent the largest family of short interspersed repetitive elements (SINEs). These mobile genetic elements are ancestrally derived from the 7SL RNA gene and move throughout the genomes of primates by retroposition. Polymorphic Alu insertions have proven to
E, Thomas, R J, Herrera
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Reaction-Mapped Quantitative Multiplexed Polymerase Chain Reaction on a Microfluidic Device
Analytical Chemistry, 2008We have applied multiple-time-point reaction mapping to generate high-dynamic-range quantitative data from PCR multiplexes. The approach measures, then compensates, numerous PCR slope nonidealities across the multiplex without prejudice. A multilane microelectophoresis device with a novel scanning detector that reports redundantly over more than six ...
Jörn, Ueberfeld +4 more
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Multiplex Quantitative Fluorescent Polymerase Chain Reaction for Detection of Aneuploidies
Genetic Testing and Molecular Biomarkers, 2012Quantitative fluorescent polymerase chain reaction (QF-PCR) is a well-established method in Western countries where commercial kits are being used for rapid prenatal diagnosis of aneuploidies. The limitations of these kits are that these are basically designed for the Caucasian population and are very expensive.
Shalu, Jain +4 more
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Multiplex polymerase chain reaction.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 1992The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced ...
L J, Burgart +5 more
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Comparison of DNA extraction methods for multiplex polymerase chain reaction
Analytical Biochemistry, 2010We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes.
Triin, Viltrop +3 more
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A Self-Priming Digital Polymerase Chain Reaction Chip for Multiplex Genetic Analysis.
ACS Nano, 2020Digital PCR (polymerase chain reaction) is a powerful and attractive tool for the quantification of nucleic acids. However, the multiplex detection capabilities of this system are limited or require expensive instrumentation and reagents, all of which ...
Juxin Yin +9 more
semanticscholar +1 more source