Results 41 to 50 of about 1,907,812 (295)

Multiplexed imaging of intracellular protein networks [PDF]

Cytometry Part A, 2016
AbstractCellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal ...
Grecco, Hernan Edgardo, Imtiaz, S., Zamir, E.   +2 more
openaire   +4 more sources

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control

Nature Communications
Optical imaging with photo-controllable probes has greatly advanced biological research. With superb chemical specificity of vibrational spectroscopy, stimulated Raman scattering (SRS) microscopy is particularly promising for super-multiplexed optical ...
Yueli Yang, Xueyang Bai, Fanghao Hu
doaj   +1 more source

Multi-modal image analysis for large-scale cancer tissue studies within IMMUcan

Cell Reports: Methods
Summary: In cancer research, multiplexed imaging allows detailed characterization of the tumor microenvironment (TME) and its link to patient prognosis. The integrated immunoprofiling of large adaptive cancer patient cohorts (IMMUcan) consortium collects
Nils Eling, Julien Dorier, Sylvie Rusakiewicz, Robin Liechti, Preethi Devanand, Michelle Daniel, Jonas Windhager, Bruno Palau Fernandez, Sophie Déglise, Lucie Despland, Abdelkader Benyagoub, Marcin Możejko, Dawid Uchal, Ewa Szczurek, Alexander Loboda, Daaf Sandkuijl, Nikesh Parsotam, Henoch S. Hong, Marie Morfouace, Nicolas Guex, George Coukos, Bernd Bodenmiller, Stephanie Tissot, Daniel Schulz   +23 more
doaj   +1 more source

Wavelength-Multiplexed Single-Shot Ptychography [PDF]

, 2020
We present multi-wavelength single-shot ptychography, a technique which is ideally suited for imaging dynamically evolving plasmas. Through improvements to single-shot ptychography and a novel probe constraint, wavelength-multiplexed single-shot ...
Jonathan Barolak, Barolak, Jonathan, Bellouard, Yves, Durfee, Charles, Adams, Daniel, Charles Durfee, Squier, Jeff, Goldberger, David, David Goldberger, Yves Bellouard, Jeff Squier, Daniel Adams   +11 more
core   +1 more source

Fluorescence Multiplexing with Spectral Imaging and Combinatorics [PDF]

ACS Combinatorial Science, 2018
ABSTRACT Ultraviolet-to-infrared fluorescence is a versatile and accessible assay modality, but is notoriously hard to multiplex due to overlap of wide emission spectra. We present an approach for fluorescence m ultiplexing
Holzapfel, Hadassa Y., Stern, Alan D., Bouhaddou, Mehdi, Anglin, Catilin M., Putur, Danielle, Comer, Sarah, Birtwistle, Marc R.   +6 more
openaire   +2 more sources

Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinoma

Molecular Oncology, EarlyView.
This study shows that lung adenocarcinomas exploit developmental branching morphogenesis to acquire a therapy resistant basal‐like tumour cell state. This process was found to be regulated by combined TP53 loss‐of‐function and type‐I interferon signalling, identifying a novel axis for biomarker and therapeutic target discovery.
Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, Emily C Shaw, Christian H Ottensmeier, Gareth J Thomas, Christopher J Hanley   +13 more
wiley   +1 more source

Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging

Nature Communications, 2021
Multiplexed ion beam imaging can provide subcellular localisation information but with limited resolution. Here the authors report an ion beam imaging method with nanoscale resolution which they use to assess the subcellular distribution of cisplatin.
Xavier Rovira-Clavé, Sizun Jiang, Yunhao Bai, Bokai Zhu, Graham Barlow, Salil Bhate, Ahmet F. Coskun, Guojun Han, Chin-Min Kimmy Ho, Chuck Hitzman, Shih-Yu Chen, Felice-Alessio Bava, Garry P. Nolan   +12 more
doaj   +1 more source

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

Molecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur, Joel Rodrigues Vitória, Manuel Saiselet, Ligia Craciun, Denis Larsimont, Anne Lefort, Frédérick Libert, Carine Maenhaut, Sabine Costagliola, Maxime Tarabichi, Mirian Romitti, Vincent Detours   +11 more
wiley   +1 more source

High-speed multiplexed DNA-PAINT imaging of nuclear organization using an expanded sequence repertoire

Nature Communications
DNA-Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) enables multiplexed super-resolution imaging of biological samples. We expand the repertoire of speed-optimized DNA-PAINT sequences to visualize up to twelve targets in a sequential ...
Abhinav Banerjee, Micky Anand, Mansi Srivastava, Vedanth Shree Vidwath, Mahipal Ganji   +4 more
doaj   +1 more source