Results 101 to 110 of about 566,949 (373)

Multiplex Pyrosequencing [PDF]

open access: yesNucleic Acids Research, 2002
We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation and hybridization of multiple sequencing primers ...
Nader, Pourmand   +3 more
openaire   +2 more sources

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

open access: yesMolecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts   +8 more
wiley   +1 more source

PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening

open access: yesBioengineering & Translational Medicine, 2020
Precise monitoring of specific biomarkers in biological fluids with accurate biodiagnostic sensors is critical for early diagnosis of diseases and subsequent treatment planning.
Xiaona Wen   +11 more
doaj   +1 more source

Diversity Order Gain with Noisy Feedback in Multiple Access Channels

open access: yes, 2008
In this paper, we study the effect of feedback channel noise on the diversity-multiplexing tradeoff in multiuser MIMO systems using quantized feedback, where each user has m transmit antennas and the base-station receiver has n antennas.
Aggarwal, Vaneet, Sabharwal, Ashutosh
core   +3 more sources

Generation of one-million-mode continuous-variable cluster state by unlimited time-domain multiplexing [PDF]

open access: yes, 2016
In recent quantum optical continuous-variable experiments, the number of fully inseparable light modes has drastically increased by introducing a multiplexing scheme either in the time domain or in the frequency domain.
J. Yoshikawa   +6 more
semanticscholar   +1 more source

Tumor‐agnostic detection of circulating tumor DNA in patients with advanced pancreatic cancer using targeted DNA methylation sequencing and cell‐free DNA fragmentomics

open access: yesMolecular Oncology, EarlyView.
We evaluated circulating tumor DNA (ctDNA) detection in advanced pancreatic cancer using DNA methylation, cell‐free DNA fragment lengths, and 5′ end motifs. Machine learning models were trained to estimate ctDNA levels from each feature and their combination.
Morten Lapin   +10 more
wiley   +1 more source

Spatial Division Multiplexing-Based Reflective Intensity-Modulated Fiber Optics Displacement Sensor

open access: yesIEEE Photonics Journal, 2018
Due to the benefit of multiple parallel channels within single cladding and easy implementation of multi-input multioutput technique, we present the spatial division multiplexing (SDM)-based reflective intensity-modulated fiber optics displacement sensor
Tianye Lan   +5 more
doaj   +1 more source

Front-end Multiplexing - applied to SQUID multiplexing : Athena X-IFU and QUBIC experiments

open access: yes, 2015
As we have seen for digital camera market and a sensor resolution increasing to "megapixels", all the scientific and high-tech imagers (whatever the wave length - from radio to X-ray range) tends also to always increases the pixels number.
Prêle, Damien
core   +3 more sources

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

open access: yesFEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli   +4 more
wiley   +1 more source

Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens

open access: yesDiagnostics
Background/Objectives: Performing hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) on the same specimen slide provides advantages that include specimen conservation and the ability to combine the H&E context with biomarker expression ...
Larry E. Morrison   +5 more
doaj   +1 more source

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