Results 91 to 100 of about 944,235 (271)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Biodegradable plastic exposure enhances microbial functional diversity while reducing taxonomic diversity across multi-kingdom soil microbiota in cherry tomato fields

open access: yesCommunications Biology
Plastic films are extensively utilized in agroecosystems, and their residues are accumulating in global soil at a worrying rate. Biodegradable films are employed as a substitute for polyethylene (PE) films due to their rapid degradation rate.
Zhen Shi   +6 more
doaj   +1 more source

Beyond IbMYB1: Identification and Characterization of Two Additional Anthocyanin MYB Activators, IbMYB2 and IbMYB3, in Sweetpotato

open access: yesPlants
Sweetpotato displays diverse purple pigmentation due to anthocyanin accumulation. While current research on the underlying MYB activators has focused on IbMYB1 in purple-fleshed tubers, the color diversity suggests the involvement of other MYB activators.
Jian Wang   +10 more
doaj   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

Effect of Cow Bone Addition on the Humification, Heavy Metals Passivation and Fate of Resistance Genes During Swine Manure Composting

open access: yesFermentation
Bone meal has been used as economic and effective additive for heavy metals (HMs) pollution remediation due to the distinct components and structures that enable their favorable properties, such as its low cost, high adsorption capacity, acid-base ...
Shanshuai Chen   +3 more
doaj   +1 more source

Antibiofilm activity of a chionodracine‐derived peptide by NMR‐based metabolomics of cell‐free supernatant of Acinetobacter baumannii clinical strains

open access: yesFEBS Open Bio, EarlyView.
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli   +9 more
wiley   +1 more source

Exploring the potential application of coconut water in healthcare and biotechnology: a review

open access: yesBeverage Plant Research
Coconut (Coconut Nucifera L.) is one of the most important palms worldwide. Coconut water, its liquid endosperm, is a nutrient-rich beverage with a wide range of potential applications as a refreshing beverage, medicine and additives in biotechnology. It
Zhihua Mu   +7 more
doaj   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

open access: yesFEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava   +3 more
wiley   +1 more source

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