Results 191 to 200 of about 40,419 (211)
Some of the next articles are maybe not open access.
Proteomic approaches to the analysis of multiprotein signaling complexes
PROTEOMICS, 2008AbstractSignal transduction is one of the most active fields in modern biomedical research. Increasing evidence has shown that signaling proteins associate with each other in characteristic ways to form large signaling complexes. These diverse structures operate to boost signaling efficiency, ensure specificity and increase sensitivity of the ...
Wei, Yang +2 more
openaire +2 more sources
Protein- and immunoaffinity purification of multiprotein complexes
2002Publisher Summary This chapter discusses the protein and immunoaffinity purification of multiprotein complexes. Virtually all proteins must interact with other proteins to carry out their activities within the cell. Protein affinity chromatography allows the exploitation of this fundamental property to identify proteins that function together as ...
Douglas R, Kellogg, Danesh, Moazed
openaire +2 more sources
Multiprotein signaling complexes and regulation of cardiac phenotype
Journal of Molecular and Cellular Cardiology, 2003There is increasing evidence across disciplines for multiprotein complexes as a mechanism for signal transduction in various cell types. These multiprotein complexes appear to be altered as a direct mechanism to confer signaling responses and to alter phenotype. Although classical experimental techniques are effective to delineate the signaling role of
Thomas M, Vondriska, Peipei, Ping
openaire +2 more sources
Catalysis by a Multiprotein IκB Kinase Complex
Science, 1997Many extracellular signals, tumor necrosis factor-a and interleukin-1 among them, transmit their signals to the genetic apparatus via the transcriptional activator, NF-kB. Maniatis summarizes what is known about this critical pathway and describes how two reports in this issue ([ Mercurio et al .][1] and [ Woronicz et al .][2]) add to the story. [1]:
openaire +2 more sources
Baculovirus expression system for heterologous multiprotein complexes
Nature Biotechnology, 2004The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes comprising many subunits.
Imre, Berger +2 more
openaire +2 more sources
The Propagation of Allosteric States in Large Multiprotein Complexes
Journal of Molecular Biology, 2013A statistical view of allostery leads to a more nuanced and physically realistic picture of protein cooperativity. If the conformational state of one protein molecule in a multiprotein complex influences the probability of a particular conformation in a neighbouring protein, then changes can propagate.
openaire +2 more sources
Mapping multiprotein complexes by affinity purification and mass spectrometry
Current Opinion in Biotechnology, 2008The combination of affinity purification and tandem mass spectrometry (MS) has emerged as a powerful approach to delineate biological processes. In particular, the use of epitope tags has allowed this approach to become scaleable and has bypassed difficulties associated with generation of antibodies. Single epitope tags and tandem affinity purification
Mark O, Collins, Jyoti S, Choudhary
openaire +2 more sources
The Cellulosome: An Exocellular, Multiprotein Complex Specialized in Cellulose Degradation
Critical Reviews in Biochemistry and Molecular Biology, 1996Clostridium thermocellum produces a highly active cellulase system that consists of a high-M(r) multienzyme complex termed cellulosome. Hydrolytic components of the cellulosome are organized around a large, noncatalytic glycoprotein termed CipA that acts both as a scaffolding component and a cellulose-binding factor.
P, Béguin, M, Lemaire
openaire +2 more sources
Analysis of Multiprotein Complexes in the Mammalian REtina
2008Eine große Gruppe genetisch vererbter Erblindungskrankheiten steht im Zusammenhang mit Mutation in Genen, die in Photorezeptoren exprimiert sind. Diese Mutationen führen nicht nur zu einer Beeinträchtigung des mutierten Proteins selbst, sondern auch zu einer Störung von funktionell nachgeschalteten Proteinnetzwerken.
openaire +1 more source
Characterisation of the MID1/alpha4 multiprotein complex
2006Mutations in the MID1 gene cause Opitz BBB/G syndrome (OS), a congenital disorder characterised by malformations of the ventral midline, with hypertelorism and hypospadias being the two cardinal phenotypic manifestations. The RBCC protein MID1 has been shown to form macromolecular complexes, the components of which were, up to now, mainly unknown.
openaire +1 more source