Results 261 to 270 of about 28,480 (301)

Cloning and expression of a cDNA encoding bovine muscarinic acetylcholine m3 receptor

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1994
A cDNA clone encoding bovine muscarinic acetylcholine m3 receptor has been isolated from a bovine brain library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 590 amino acids. Comparison with known muscarinic m3 receptor sequences from other species shows a high degree of conservation (92-98% homology) in the ...
Paula K. Hodges, Fraser Glickman, Kwen-Jen Chang, Paul H.K. Lee   +3 more
openaire   +3 more sources

Determination of Region-Specific Roles of the M3 Muscarinic Acetylcholine Receptor in Gastrointestinal Motility

Digestive Diseases and Sciences, 2022
The specific role of the M3 muscarinic acetylcholine receptor in gastrointestinal motility under physiological conditions is unclear, due to a lack of subtype-selective compounds.The objective of this study was to determine the region-specific role of the M3 receptor in gastrointestinal motility.We developed a novel positive allosteric modulator (PAM ...
Yoko Igarashi-Hisayoshi, Eikichi Ihara, Xiaopeng Bai, Chika Higashi, Hiroko Ikeda, Yoshimasa Tanaka, Mayumi Hirano, Haruei Ogino, Takatoshi Chinen, Yasushi Taguchi, Yoshihiro Ogawa   +10 more
openaire   +2 more sources

Interaction of Edrophonium with Muscarinic Acetylcholine M2and M3Receptors

Anesthesiology, 2001
Background It has been reported that edrophonium can antagonize the negative chronotropic effect of carbachol. This study was undertaken to evaluate in detail the interaction of edrophonium with muscarinic Mz and M3 receptors. Methods A functional study was conducted to evaluate the
Yasuto Tanito, Yoshihumi Hirose, Masayuki Endou, Takaaki Miwa, Haruaki Nakaya, Masahiro Gamoh, Fukuichiro Okumura   +6 more
openaire   +3 more sources

The role of M3 muscarinic acetylcholine receptor reactive T cells in Sjögren's syndrome: a critical review.

Journal of Autoimmunity, 2014
CD4+ T cells constitute the majority of infiltrating cells in salivary glands and lachrymal glands of patients with Sjögren's syndrome (SS). The pathophysiology of SS involves T cell recognition of antigens through the T cell antigen receptor, which triggers cytokine production and chronic inflammation.
T. Sumida, H. Tsuboi, M. Iizuka, T. Hirota, H. Asashima, I. Matsumoto   +5 more
semanticscholar   +3 more sources

M3 muscarinic acetylcholine receptor dysfunction inhibits Rac1 activity and disrupts VE-cadherin/β-catenin and actin cytoskeleton interaction.

Biochemistry and cell biology = Biochimie et biologie cellulaire, 2014
The objective was to investigate whether M3 muscarinic acetylcholine receptor (mAChR) dysfunction disrupts the linkage between the vascular endothelial (VE)-cadherin in the adherens junctional complex and the actin-based cytoskeleton, increasing vascular
Zhou-yang Jiao, Jing Wu, B. Wen, Wen-zeng Zhao, Xin-ling Du   +4 more
semanticscholar   +1 more source

M3 muscarinic acetylcholine receptor antagonists: SAR and optimization of bi-aryl amines

Bioorganic & Medicinal Chemistry Letters, 2009
Exploration of multiple regions of a bi-aryl amine template led to the identification of highly potent M(3) muscarinic acetylcholine receptor antagonists such as 14 (pA(2)=11.0) possessing good sub-type selectivity for M(3) over M(2). The structure-activity relationships (SAR) and optimization of the bi-aryl amine series are described.
Ralph A. Rivero, Yonghui Wang, Henry M. Sarau, Michael Salmon, Zehong Wan, Chongye Zhu, Feng Wang, Dongchuan Shi, Dwight M. Morrow, Palovich Michael R, Brian Budzik, James J. Foley, Kristen E. Belmonte, Lorena A. Kallal, Dramane I. Laine, Parvathi Nuthulaganti, Haibo Xie, Michael L. Moore, Jian Jin   +18 more
openaire   +3 more sources

Role of microtubule structure in the maintenance of m3-muscarinic acetylcholine receptor rnRNA levels

Molecular and Cellular Neuroscience, 1991
The regulation of m3-muscarinic acetylcholine receptor (mAChR) mRNA following exposure to microtubule-disrupting agents was studied in cultured cerebellar granule cells. Colchicine (10 muM), an antimicrotubular agent, decreased the levels of m3-mAChR mRNA in a time-dependent fashion.
De-Maw Chuang, Fumihiko Fukamauchi, Christopher Hough   +2 more
openaire   +3 more sources

M3 muscarinic acetylcholine receptor coupling to PLC in rat exorbital lacrimal acinar cells

American Journal of Physiology-Cell Physiology, 1993
This study was designed to characterize the muscarinic acetylcholine receptor (mAChR) subtype present in rat exorbital lacrimal gland as well as its biochemical coupling. The nonselective muscarinic antagonist [N-methyl-3H]scopolamine ([3H]NMS) binds with high affinity to a homogeneous population of binding sites in both membranes [dissociation ...
Mauduit, P., Jammes, Hélène, Rossignol, B.   +2 more
openaire   +4 more sources

Muscarinic Stimulation of Pancreatic Insulin and Glucagon Release Is Abolished in M3 Muscarinic Acetylcholine Receptor–Deficient Mice

Diabetes, 2004
Pancreatic muscarinic acetylcholine receptors play an important role in stimulating insulin and glucagon secretion from islet cells. To study the potential role of the M3 muscarinic receptor subtype in cholinergic stimulation of insulin release, we initially examined the effect of the muscarinic agonist, oxotremorine-M (Oxo-M), on insulin secretion ...
Alokesh Duttaroy, Dinesh Gautam, Jürgen Wess, Charles L. Zimliki, David Mears, Yinghong Cui   +5 more
openaire   +3 more sources

Differential calcium signalling by m2 and m3 muscarinic acetylcholine receptors in a single cell type

Naunyn-Schmiedeberg's Archives of Pharmacology, 1995
We have compared muscarinic acetylcholine receptor (mAChR) coupling to phospholipase C (PLC) and increases in cytoplasmic Ca2+ concentration [Ca2+]i in human embryonic kidney (HEK) cells, stably expressing either the human m3 or m2 receptor subtype. In m3 mAChR-expressing cells, carbachol stimulated inositol phosphate (InsP) formation and increased ...
Martina Schmidt, Karl Jakobs, Martin C. Michel, C. J. Van Koppen, Christine Bienek   +4 more
openaire   +3 more sources