Results 151 to 160 of about 14,921,471 (288)

Protocol for quantification of UDP-GlcNAc using an enzymatic microplate assay

STAR Protocols
Summary: Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) is the end product of the hexosamine biosynthetic pathway and the substrate for protein O-linked N-acetylglucosaminylation (O-GlcNAcylation). Here, we present a protocol for the quantification
Divya Upadhyay, Rishi Banerjee, Jukka Kallijärvi, Janne Purhonen   +3 more
doaj   +1 more source

Interactions of Mucus Monosaccharides and the Epidermal Microbiome in Four Benthic Elasmobranchs

Environmental Microbiology Reports, Volume 18, Issue 2, April 2026.
Here we collected mucus and microbes from the skin of Elasmobranchs to identify interactions between mucus and the microbial community. We identified key microbial genes involved in the metabolism of mucus carbohydrates in Elasmobranchs. ABSTRACT Epidermal mucus is a complicated mixture of macromolecules which acts as the first line of defence for ...
Emma N. Kerr, Long Yu, Ryan D. Hesse, Chloe N. Roberts, Vincent Bulone, Lauren Meyer, Robert A. Edwards, Michael P. Doane, Elizabeth A. Dinsdale   +8 more
wiley   +1 more source

Aberrant N-glycosylation may be a therapeutic target in carriers of a common and highly pleiotropic variant in the manganese transporter ZIP8

HGG Advances
Summary: The treatment of defective glycosylation in clinical practice has been limited to patients with rare and severe phenotypes associated with congenital disorders of glycosylation (CDGs).
Vartika Tomar, John Kang, Ruxian Lin, Steven R. Brant, Mark Lazarev, Caitlin Tressler, Kristine Glunde, Natasha Zachara, Joanna Melia   +8 more
doaj   +1 more source

Requirement of group I lytic polysaccharide monooxygenase for turnover of chitinous cuticle during moulting in two forest pest beetles, Monochamus alternatus and Psacothea hilaris

Insect Molecular Biology, Volume 35, Issue 2, Page 115-125, April 2026.
Group I LPMO15‐1 cDNAs from two economically important forest insect pests, M. alternatus and P. hilaris, were cloned. MaLPMO15‐1 and PhLPMO15‐1 show a similar pattern of expression during late stages of development. RNAi for LPMO15‐1 causes failure of adult eclosion in both M. alternatus and P. hilaris.
Daehyeong Kim, Subbaratnam Muthukrishnan, Karl J. Kramer, Yasuyuki Arakane, Mi Young Noh   +4 more
wiley   +1 more source

Glycolytic enzymes - novel carbohydrate binding proteins for glycoprotein analysis [PDF]

, 2011
•The cloning, expression, purification and characterisation of recombinant prokaryotic glycolytic enzymes •The mutagenesis of prokaryotic glycolytic enzymes to generate novel recombinant carbohydrate binding proteins •The characterisation of the ...
Clarke, Paul A., Harrington, Amy, O'Connor, Brendan, Thompson, Roisin   +3 more
core  

Regulation of pyruvate dehydrogenase complex: Dancing to different drums in cancer

International Journal of Cancer, Volume 158, Issue 6, Page 1464-1480, 15 March 2026.
Abstract Mechanisms governing the regulation of pyruvate dehydrogenase complex (PDC) are markedly modified in cancer cells compared to normal cells. PDC activity in normal cells is controlled by the reversible phosphorylation of three serine residues by dedicated kinases and phosphatases.
Mulchand S. Patel, Todd C. Rideout
wiley   +1 more source

Rational Design of Optical Single‐Walled Carbon Nanotube‐Based Nanosensors with Biological Recognition Elements

Advanced Sensor Research, Volume 5, Issue 3, March 2026.
This Review focuses on assessing and providing perspective on the field of rationally‐designed optical sensors constructed with single‐walled carbon nanotubes. The literature is reviewed and evaluated for SWCNT‐based sensors constructed with biomolecular recognition elements, including proteins, peptides, and oligonucleotides, as well as their methods ...
Amelia K. Ryan, Atara Israel, Maria Celina Stefoni, Catarina Ferraz, Ryan M. Williams   +4 more
wiley   +1 more source

Enzymatic Remodelling of Tumour Microenvironment Enhances Anti‐CEACAM5 CAR T‐Cell Efficacy Against Colorectal Cancer

Advanced Science, Volume 13, Issue 13, 3 March 2026.
This study shows anti‐CEACAM5 CAR T‐cells are ineffective against colorectal cancer (CRC) because of CEACAM5 sequestration at intercellular junctions and the thick tumour cell glycocalyx. Enzymatic treatments of CRC cell monolayer/tissue section with trypsin or hyaluronidase restore CEACAM5 availability, enhance CAR T‐cell activation, increase ...
Debasis Banik, Christopher J. Ward, Soura Chakraborty, Ziwei Zhang, Daniel Heraghty, Prasanna Suresh, Bing Li, Shekhar Kedia, Jhuma Pramanik, Simon J. Davis, James P. Roy, Michael A. Chapman, Bidesh Mahata, David Klenerman   +13 more
wiley   +1 more source

Electric Method for the Desulfation of Polysaccharides From Red Microalgae (Porphyridium cruentum) Cultures

Biopolymers, Volume 117, Issue 2, March 2026.
A non‐destructive method for depleting sulfates from the red microalgae Porphyridium cruentum by directly exposing the growth medium to a moderate electric field has been developed. The reported electric‐field separation method is a simple, straightforward, and non‐toxic means for the direct recovery of desulfated polysaccharides from P.
Oshrat Levy‐Ontman, Amikam Bar‐Gil, Eli Shemesh, Mahmoud Huliehel, Yehudit Amor, Naama Landes, Yonatan Sadeh, Dan Tchernov   +7 more
wiley   +1 more source

Role of O-linked N-acetylglucosamine modification in diabetic nephropathy.

AJP - Renal Physiology, 2016
Renata Gellai, J. Hodrea, Lilla Lenart, Á. Hosszú, Sándor Kőszegi, D. Balogh, Á. Vér, N. Bánki, Norbert Fulop, A. Molnar, L. Wagner, Á. Vannay, A. Szabó, Andrea Fekete   +13 more
semanticscholar   +1 more source