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Axenic cultivation of Naegleria gruberi
Experimental Cell Research, 1974Abstract A simplified axenic medium for Naegleria gruberi strain NEG-M contains l -methionine, dextrose, yeast extract, a macromolecular fraction of fetal calf serum, and phosphate buffer. Amoebae cultured in suspension in this medium grow with doubling times of 8–10 h (at 32 °C) to yield 2–4 × 10 6 cells/ml.
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Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA
Nature, 2013Hideharu Hashimoto +2 more
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