Results 191 to 200 of about 3,607 (224)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Nature's Master of Ceremony: The <i>Populus</i> Circadian Clock as Orchestratot of Tree Growth and Phenology. [PDF]

NPJ Biol Timing Sleep
Mariën B, Robinson KM, Jurca M, Michelson IH, Takata N, Kozarewa I, Pin PA, Pin PA, Ingvarsson PK, Moritz T, Ibáñez C, Nilsson O, Jansson S, Penfield S, Yu J, Eriksson ME.   +15 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

An Aboriginal women-led approach to design a maternal and child health model when cardiometabolic complications are experienced in pregnancy in South Australia. [PDF]

Med J Aust
DeMasi Barkandji K, Shen Ngarrindjeri D, McColl P, Richards-Satour Adnyamathanha And Barngarla A, Renehan Central Arrernte C, Morey Anmatyerr And Eastern Arrernte K, Glover Mein Tnk And Wotjobaluk K, Leane Dharug C, Woods-Hampton Anmatijerre K, Garay Anangu L, Baker Adnyamathanha And Barngarla E, Nielsen Kalkadoon R, Brown K.   +12 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

The relationship between Rose Anna Shedlock (c1850-1878) and Emile Roux (1853-1933). [PDF]

J Med Biogr
Murnane JP, Probert R.
europepmc   +1 more source

The Gendered, Misogynoiristic, and Colonial Genocidal Logics of Strip Searching. [PDF]

Affilia
Hutchison J.
europepmc   +1 more source

Storylines of family medicine VI: ways of being-in the office with patients. [PDF]

Fam Med Community Health
Ventres WB, Stone LA, LaVallee LA, Loxterkamp D, Brown JR, Waxman DM, Dorward PS, Cawse-Lucas J, Mauksch LB, Kieber-Emmons AM, Crabtree BF, Miller WL, Brohm VM, Daaleman TP, Bossenbroek Fedoriw K.   +14 more
europepmc   +1 more source

Institutionalized violence in schools and language displacement: the voices of Mapuche speakers and elders. [PDF]

Front Psychol
Sanhueza S, Maldonado F, Díaz C, Friz M, Toloza CA, Cuevas HT.   +5 more
europepmc   +1 more source