Results 141 to 150 of about 5,275 (178)

NanoLuc as a tool to study infection with a plant virus

, 2018
openaire   +1 more source

Bright calcium-modulated bioluminescent indicators for activity imaging and red photon-assisted synaptic transmission

Zhao Y, Porta-de-la-Riva M, Lee S, Wu Y, Klein M, Hall MP, Su Y, Encell LP, Kirkland TA, Krieg M, Lin MZ.   +10 more
europepmc   +1 more source

De novo design of protein competitors for small molecule immunosensing

de Stigter Y, Godschalk T, Merkx M.
europepmc   +1 more source

Intracellular Ionic Strength Sensing Using NanoLuc [PDF]

International Journal of Molecular Sciences, 2021
Intracellular ionic strength regulates myriad cellular processes that are fundamental to cellular survival and proliferation, including protein activity, aggregation, phase separation, and cell volume. It could be altered by changes in the activity of cellular signaling pathways, such as those that impact the activity of membrane-localized ion channels
Tausif Altamash, Kabir Biswas, Wesam S Ahmed   +2 more
exaly   +3 more sources

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Caspase sensors based on NanoLuc

Journal of Biotechnology, 2022
Caspases are a family of evolutionary conserved cysteine proteases that play key roles in programmed cell death and inflammation. Among the methods for the detection of caspase activity, biosensors based on luciferases have advantages in genetical encoding and convenience in assay.
Jie, Li, Jin-Lan, Wang, Chun-Yang, Gan, Xue-Fei, Cai, Yu-Wei, Wang, Quan-Xin, Long, Yu-Xue, Sun, Xia-Fei, Wei, Jing, Cui, Ai-Long, Huang, Jie-Li, Hu   +10 more
openaire   +2 more sources

Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay [PDF]

Frontiers in Microbiology, 2021
Studies of biofilm formation by bacteria are crucial for understanding bacterial resistance and for development of novel antibacterial strategies. We have developed a new bioluminescence biofilm assay for Listeria innocua, which is considered a non-pathogenic surrogate for Listeria monocytogenes. L.
Jerica Sabotič, Meta Sterniša, Ales Berlec   +2 more
exaly   +4 more sources

A NanoLuc-Based Transactivation Assay in Plants

2023
Protein-DNA interactions are determinant of the regulation of gene expression in living organisms. Luminescence studies have been used in a wide range of techniques to identify how gene transcription can be regulated by proteins such as transcription factors (TFs).
Rosa Esmeralda, Becerra-García, José Erik, Cruz-Valderrama, Vincent E, Cerbantez-Bueno, Nayelli, Marsch-Martínez, Stefan, de Folter   +4 more
openaire   +2 more sources

Engineering with NanoLuc

Biochemical Society Transactions, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Biewenga, Lieuwe, Rosier, Bas J.H.M., Merkx, Maarten   +2 more
openaire   +1 more source

Novel NanoLuc-type substrates with various C-6 substitutions

Bioorganic and Medicinal Chemistry Letters, 2020
NanoLuc (NLuc)-furimazine bioluminescence system offers several advantages over established systems, including improved stability, smaller size, and >150-fold enhancement in bioluminescence. Herein, we designed and synthesized a series of bioluminescent substrates with varying at the C-6 position of furimazine for NLuc-furimazine bioluminescence system.
Lupei Du, Minyong Li
exaly   +3 more sources