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Nanopore sequencing technology: nanopore preparations

Trends in Biotechnology, 2007
For the past decade, nanometer-scale pores have been developed as a powerful technique for sensing biological macromolecules. Various potential applications using these nanopores have been reported at the proof-of-principle stage, with the eventual aim of using them as an alternative to de novo DNA sequencing.
Minsoung, Rhee, Mark A, Burns
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The Nanopore Electrode

Analytical Chemistry, 2004
The fabrication and electrochemical characterization of truncated cone-shaped nanopore electrodes are reported. A nanopore electrode is a Pt disk electrode embedded at the bottom of a conical pore, the circular orifice of the pore having nanometer dimensions.
Bo, Zhang, Yanhui, Zhang, Henry S, White
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Nanopore Cheminformatics

DNA and Cell Biology, 2004
A cheminformatics method is described for classification, and biophysical examination, of individual molecules. A novel molecular detector is used--one based on current blockade measurements through a nanometer-scale ion channel (alpha-hemolysin). Classification results are described for blockades caused by DNA molecules in the alpha-hemolysin nanopore
Stephen, Winters-Hilt, Mark, Akeson
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Fluorescence biosensing in nanopores

2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2009
Hydrated nanopores offer a unique environment for studying biological molecules under controlled conditions and fabricating sensors using fluorescence. Silica nanopores for example are non-toxic, biologically and optically compatible with protein, and can be easily synthesized to entrap protein and exclude potentially interfering macromolecules, while ...
Karolin, Jan   +4 more
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A nanopore workhorse

Nature Methods, 2012
Voltage-driven and enzyme-regulated control allows precise ratcheting of a DNA strand in and out of a nanopore.
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Protein conducting nanopores

Journal of Physics: Condensed Matter, 2010
About 50% of the cellular proteins have to be transported into or across cellular membranes. This transport is an essential step in the protein biosynthesis. In eukaryotic cells secretory proteins are transported into the endoplasmic reticulum before they are transported in vesicles to the plasma membrane.
Harsman, Anke   +7 more
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Adsorbate Transport in Nanopores

Adsorption, 2005
We present a tractable theory of transport of simple fluids in cylindrical nanopores, considering trajectories of molecules between diffuse wall collisions at low-density, and including viscous flow contributions at higher densities. The model is validated through molecular dynamics simulations of supercritical methane transport, over a wide range of ...
Bhatia, SK, Jepps, OG, Nicholson, D
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