Results 71 to 80 of about 2,046,661 (306)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The Effects of Foreign Government Policies on the Location of U.S. Direct Investment Abroad
East Asian Economic Review, 2001 Many argue about which policy and non-policy variables play an important role in influencing foreign direct investment decisions. This article contributes to this debate by considering the effects of foreign government policies on the location of U.S ...
Kee Min
doaj +1 more source
Legislative drafting learning process in Mozambique [PDF]
, 1995 Documentation from Robert and Ann Seidman on their series of 1996 workshops on setting up a learning process in Mozambique on legislative drafting theory and methodology.
Seidman, Ann, Seidman, Robert B.
core +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Nature Communications, 2018 Many aspects of concentrated macromolecular solutions, such as encountered in cytosol or in pharmaceutical formulations, are dependent on particle size distributions and weak intermolecular interactions.
Sumit K. Chaturvedi +4 more
doaj +1 more source
Education Bill: provisions relating to Wales [PDF]
, 2011 This is a background briefing on the Education Bill. "The Education Bill was presented to the UK Parliament by the Rt. Hon Michael Gove MP, the Secretary of State for Education, on 26 January 2011.
Roberts, Owain
core
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Transforming Masculinist Political Cultures? Doing Politics in New Political Institutions [PDF]
In the devolved legislative assemblies of Scotland and Wales the proportion of women representatives is approaching parity. This is in marked contrast to Westminster where one in five MPs are women.
Charlotte Aull Davies +2 more
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Westminster can learn a lot about gender equality by looking at Welsh and Scottish levels of political representation [PDF]
, 2012 Discrimination and under-representation of women is still overt and visible in politics. Diana Silvia Stirbu argues that progress on the gender equality agenda doesn’t come easily, but it is possible through a combination of structural, political and ...
Stirbu, Diana Silvia
core
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source