Results 111 to 120 of about 4,554,835 (278)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

On the decidability of connectedness constraints in 2D and 3D Euclidean spaces [PDF]

, 2011
We investigate (quantifier-free) spatial constraint languages with equality, contact and connectedness predicates as well as Boolean operations on regions, interpreted over low-dimensional Euclidean spaces. We show that the complexity of reasoning varies
Yavor Nenov, Pratt−Hartmann, Ian, Ian Pratt−Hartmann, Roman Kontchakov, Pratt-Hartmann, Ian; id_orcid, Ian Pratt-hartmann, Michael Zakharyaschev, Pratt-Hartmann, I., Nenov, Y., Zakharyaschev, Michael, Nenov, Yavor, Kontchakov, Roman   +11 more
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Nature nurtures our wellbeing: primary emotions and attachment mediate our psychological adjustment via connectedness to nature

Frontiers in Cognition
IntroductionResearch suggests that the psychological benefits of natural environments are not uniformly experienced, with individual differences in nature connectedness potentially shaped by temperament traits and attachment patterns moderating their ...
Amadeu Quelhas Martins, Amadeu Quelhas Martins, Amadeu Quelhas Martins, Júlia Fonte, Júlia Fonte, Nuno Torres, Paulo Ferrajão, Paulo Ferrajão   +7 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Impact of Web technologies on student-lecturer connectedness [PDF]

In academic sense connectedness is recognized as students’ active engagement in the academic and social opportunities at their school based on their understanding that teachers at school care for them as individuals, as well as for their learning ...
Alshahrani, Saeed, Ward, Rupert
core   +3 more sources

The sense of connection with nature among leaders of thematic villages in Eastern Poland

Prace Geograficzne
The aim of this study was to determine whether individuals engaged in the development of thematic villages experience a sense of connectedness to nature.
Natalia Korcz, Agata Kobyłka, Anna Katarzyna Mazurek-Kusiak, Ryszarda Ewa Bernacka   +3 more
doaj   +1 more source