Results 221 to 230 of about 3,105,218 (296)

Protocol for DRIFT-EM: Direct wafer retrieval of ultrathin serial sections for volume electron microscopy. [PDF]

STAR Protoc
Gogola JV, Leo Justin A, Wildenberg G.
europepmc   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

High-yield charge separation along the alternative path in a photosynthetic reaction center: X-ray structure and electrostatic analysis. [PDF]

Proc Natl Acad Sci U S A
Keable SM, Wei RJ, Buhrmaster JC, Hippleheuser S, Makita H, Simon PS, Tira GA, Kretzschmar M, Bogacz I, Faries KM, Lan A, Nangca II, Zhang M, Doyle MD, Chernev P, Bhowmick A, Paley DW, Sauter NK, Brewster AS, Tono K, Owada S, Yano J, Yachandra VK, Gunner MR, Kirmaier C, Holten D, Hanson DK, Laible PD, Kern JF.   +28 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Snapshots From the Catalytic Landscape of Chalcone Isomerase. [PDF]

Plant Direct
Burke JR, Rangel MA, Vasquez Meza VI, Mims HN, Darko TO, Ixcoy K, Rivera AR, Zhang J, Wolf-Saxon ER, Moorman CC.   +9 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Broadband solar energy harvesting and near-perfect thermal emission on a unified stepped-high concentric dual-ring metamaterial. [PDF]

Sci Rep
Liang Q, Gu Y, Wu S, Chen J, Chen X.
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source