Results 41 to 50 of about 461,376 (298)

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

open access: yesFEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li   +10 more
wiley   +1 more source

Accumulation of heavy metals in needles and bark of Pinus species

open access: yes, 2017
During the research, cumulative properties of conifer needles P. armandii, P. banksiana, P. mugo, P. nigra, P. sylvestris and P. wallichiana in reference to Cu, Ni, Mn, Fe, Zn and Cd were analysed, and the factors which have an impact on the chemical ...
A. Parzych   +4 more
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

ELEMENTAL COMPOSITION OF SCOTS PINE WOOD, BARK AND NEEDLES IN THE MARI EL FORESTS [PDF]

open access: yesВопросы лесной науки
The article presents the results of assessment of Scots pine (Pinus sylvestris L.) wood (216 pc), bark (29 pc), needle (127 pc) specimens for the content of 13 chemicals (Ca, K, Mg, Mn, Zn, Fe, Cu, Sr,Ni,Pb, Cr, Co, Cd), which were tested using an ...
Yu. P. Demakov   +2 more
doaj   +1 more source

INF2‐Related Charcot–Marie–Tooth Disease in a Japanese Cohort: Genetic and Clinical Insights

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background INF2 mutations cause focal segmental glomerulosclerosis (FSGS) and Charcot–Marie–Tooth disease (CMT). Accurate genetic diagnosis is critical, as INF2‐related FSGS is typically resistant to immunotherapy yet rarely recurs after transplantation, and its associated neuropathy can mimic treatable immune‐mediated disorders such as ...
Chikashi Yano   +27 more
wiley   +1 more source

Nationwide Survey of Atopic Myelitis and Plexin D1‐Immunoglobulin G‐Related Pain

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To elucidate the features of plexin D1‐immunoglobulin (Ig)G‐associated neuropathic pain and its relationship to atopic myelitis (AM) in a nationwide Japanese survey. Methods A preliminary survey questionnaire was sent to 1574 selected departments (neurology and pediatrics/pediatric neurology) to explore the numbers of AM and plexin ...
Jun‐ichi Kira   +10 more
wiley   +1 more source

Development and face validation of ultrasound-guided renal biopsy virtual trainer

open access: yesHealthcare Technology Letters, 2019
The overall prevalence of chronic kidney disease in the general population is ∼14% with more than 661,000 Americans having a kidney failure. Ultrasound (US)-guided renal biopsy is a critically important tool in the evaluation and management of renal ...
Andinet Enquobahrie   +5 more
doaj   +1 more source

Basilar Artery Occlusion Stroke Managed With Tenecteplase Versus Alteplase Before Endovascular Treatment (BAO‐TNK)

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To compare the effectiveness and safety of tenecteplase (TNK) versus alteplase (TPA) in patients with basilar artery occlusion prior to endovascular treatment (EVT). Methods In this retrospective multicenter study (BAO‐TNK), we analyzed consecutive BAO patients from 14 U.S.
Rahul R. Karamchandani   +38 more
wiley   +1 more source

Morphological and functional features of Scots pine in pine forest stands of the Small Polissia

open access: yesНаукові праці Лісівничої академії наук України, 2019
The paper examines the formation and morphophysiological features of functioning of 27-145-year-old pine forest stands of natural seed origin which grow in dry, fresh, moist, wet, and very wet pine forests of Small Polissia.
Volodymyr Zaika , Anatoliy Rudenko
doaj   +1 more source

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