Results 211 to 220 of about 2,274,380 (266)

How loneliness impacts depression among Chinese college students throughout COVID-19: mediators of death anxiety and negative affect. [PDF]

Front Psychiatry
Lv F, Feng R, Tan J, Li J, Liu Y, Gao D.
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

NREM Sleep Oscillations Are Associated With Anxiety and Negative Affect in Young Adults. [PDF]

J Sleep Res
Arpaci H, Banik N, Ersoy PK, Harrington C, Kapucu A, Baran B.   +5 more
europepmc   +1 more source

Can kinesio tape negatively affect the treatment by creating a hard floor in plantar fasciitis treatment? A randomized clinical trial

Tuğba Kocahan, Aydan Örsçelik, Hüseyin Günaydın, Gökhan Büyüklüoğlu, Burak Karaaslan, Erdoğan Asar, Bihter Akınoğlu   +6 more
openalex   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

The moderating role of negative affect on pain in patients with rheumatoid arthritis who have undergone shoulder arthroplasty. [PDF]

J Orthop Surg Res
Asimina L, Tim S, Daniela B, Robert E, Markus S.   +4 more
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Mindfulness and Relaxation Interventions Reduce Depression, Negative Affect and Stress in Widow(er)s. [PDF]

J Loss Trauma
Knowles LM, Kaplan DM, Flores M, Friedman SE, O'Connor MF.   +4 more
europepmc   +1 more source

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

FEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner, César G. Prucca, Lucia Boffelli, Virginia A. Piqueras, Silvia G. Correa, Mariana Maccioni, Mario E. Guido   +6 more
wiley   +1 more source