Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Microscopic mechanism of anyon superconductivity emerging from fractional Chern insulators.
Pichler F +3 more
europepmc +1 more source
Ground-state and finite-temperature phase diagrams of the Bose-Hubbard model under artificial magnetic fields. [PDF]
Jaberi M, Heydarinasab F.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Entropic Dynamics Approach to Quantum Electrodynamics. [PDF]
Caticha A.
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Evaluating GNSS infrastructure readiness for IGS contribution: the case of King Sejong Station, Antarctica. [PDF]
Lee SJ, Yun HS, Yoon HM, Lee SH.
europepmc +1 more source
Gauge-boson self-interactions and tt production as probes of new physics at high energy e+e- colliders. [PDF]
openaire +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Matrix Quantum Mechanics and Entanglement Entropy: A Review. [PDF]
Fliss JR, Frenkel A.
europepmc +1 more source

