Results 51 to 60 of about 149,134 (260)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Education Policy Analysis Archives, 2015 Based on a school ethnography carried out in an English primary school in the last years of New Labour Government, this paper examines the processes and dynamics involved in the introduction of a cooperative learning method as part of the Success for ...
Laura Luna Figueroa
doaj +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Disentangling (new) labour market divides: outsiders' and globalization losers' socio-economic risks in Europe. [PDF]
Qual Quant, 2023 Natili M, Negri F.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
The multistage nature of labour migration from Eastern and Central Europe (experience of Ukraine, Poland, United Kingdom and Germany during the 2002-2011 period) [PDF]
Eastern Journal of European Studies, 2015 This article examines the consequences of the biggest round of EU Enlargement in 2004 on the labour migration flows from the new accession countries (A8) of the Eastern and Central Europe to Western Europe.
Khrystyna FOGEL
doaj
Will Work For Free: The Biopolitics of Unwaged Digital Labour
tripleC: Communication, Capitalism & Critique, 2014 This paper begins with a survey of the literature regarding a particular, yet ever more consequential and profitable, typology of digital labour: ‘free labour’ (Terranova, 2000, 2004), ‘unwaged immaterial labour’ (Brown and Quan Haase, 2012; Brown, 2013),
Brian Brown
doaj +1 more source
From Rana Plaza to COVID-19: Deficiencies and opportunities for a new labour governance system in garment global supply chains. [PDF]
Int Labour Rev, 2021 Frenkel SJ, Schuessler ES.
europepmc +1 more source
FEBS Open Bio, EarlyView.The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley +1 more source