Results 181 to 190 of about 5,260,738 (315)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Developing new technologies to protect ecosystems: Planning with adaptive management. [PDF]

Proc Natl Acad Sci U S A
Pascal LV, Chadès I, Adams MP, Helmstedt KJ.   +3 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Integrating New Technologies in Lipidology: A Comprehensive Review. [PDF]

J Clin Med
Escobar-Cervantes C, Saldaña-García J, Torremocha-López A, Contreras-Lorenzo C, Lara-García A, Canales-Muñoz L, Martínez-González R, Vila-García J, Banach M.   +8 more
europepmc   +1 more source

Eisenstadt, The New Reproductive Technologies and the Single Parent [PDF]

, 1986
Riga, Peter J.
core   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Perceived usefulness of new technologies in palliative care volunteering: mixed-methods study with stakeholders. [PDF]

BMC Palliat Care
Barnestein-Fonseca P, Víbora-Martín E, Ruiz-Torreras I, Gómez-García R, Martín-Roselló ML.   +4 more
europepmc   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Energies and new technologies in pelvic and pelvic floor dysfunctions. [PDF]

Rev Bras Ginecol Obstet
Campaner AB, Valadares ALR, Vale FBC, de Oliveira LM, Schreiner L, Lara LAS, Costa-Paiva L, Sartori MGF, Speck NMG, Bella ZIKJD.   +9 more
europepmc   +1 more source

New Technologies in High-Speed Wireless Information Transfer [PDF]

, 2014
Hovorukha, Yaroslav, Ivanchenko, I. A., Ogeyenko, P. Yu.   +2 more
core