Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Multilingual news dataset about Ukraine (2022-2025): data collection and documentation. [PDF]
Lipianina-Honcharenko K +4 more
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
How to systematically and quantifiably remove meaning? [PDF]
Proschinger Åström F, Hintze A.
europepmc +1 more source
ChunkyBERT: a novel technique for multiclass political bias detection in news media. [PDF]
Loiya D, Kulal SS, Reddy MSM, Shetty RD.
europepmc +1 more source
Clinical Partnering - When Should Host Nation Patients Not be Directed to Coalition Military Hospitals? [PDF]
Bricknell M.
europepmc +1 more source
Exploring Attitudes to Lung Cancer Screening in England: An Inductive Content Analysis of Online Commentary Following Media Announcement of a National Lung Cancer Screening Programme. [PDF]
Milne A, Chalmers J, Quaife SL, Bibby A.
europepmc +1 more source
Disinformation, Psychosocial Vulnerability, and Media Trust in the Digital Era: Implications for Health Behaviour and Societal Resilience. [PDF]
Alves Ferreira JM +2 more
europepmc +1 more source

