Results 131 to 140 of about 71,620 (303)

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

open access: yesNew Phytologist, EarlyView.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng   +4 more
wiley   +1 more source

Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15‐mediated unloading of sucrose from the host's phloem

open access: yesNew Phytologist, EarlyView.
Cuscuta reflexa infection sites. Summary Cuscuta species are holoparasitic plants that penetrate host stems with haustoria, connect to the vasculature and exhaust water, nutrients and carbohydrates. Parasite seedlings possess only limited maternal reserves and the development of a vascular connection takes several days.
Maleen Hartenstein   +10 more
wiley   +1 more source

Cross‐stage single‐cell and spatial metabolome analyses reveal periderm specialization and tanshinone biosynthesis in Salvia miltiorrhiza roots

open access: yesNew Phytologist, EarlyView.
Biosynthesis and accumulation of tanshinones in the periderm of Salvia miltiorrhiza during secondary root growth. Summary Perennial herbs develop long‐lived roots that undergo repeated cycles of secondary growth, during which the periderm functions as a protective barrier and a major site of specialized metabolite accumulation.
Licheng Liu   +19 more
wiley   +1 more source

Influence of viral genes on the cell-to-cell spread of RNA silencing [PDF]

open access: yes
The turnip crinkle virus-based vector TCV–GFPDCP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing.
Hong, Yiguo   +3 more
core   +1 more source

Barley HvBODYGUARD1 controls cuticular specialisations regulated by SHINE transcription factors

open access: yesNew Phytologist, EarlyView.
Cuticle defects result from defective HvBDG1 alleles. Summary Land plants secrete a protective outer cuticular layer with diverse functions. Barley (Hordeum vulgare L.) develops two cuticular specialisations: the β‐diketone rich wax bloom on vegetative tissues and an adherent grain surface which sticks to the hulls, leading to barley's distinctive ...
Trisha McAllister   +19 more
wiley   +1 more source

Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding

open access: yesNew Phytologist, EarlyView.
Strategies for introducing genome edits into a wheat breeding program and their effects on cultivar longevity. Summary Bread wheat (Triticum aestivum) production is increasingly threatened by biotic and abiotic stresses. Developing varieties with improved stress tolerance and desirable end‐use qualities is crucial for meeting growing global demand ...
Abdulrahman Alhabsi   +7 more
wiley   +1 more source

Development of identification method for Nicotiana sylvestris, Nicotiana tabacum, and Nicotiana rustica species for forensic expertise purposes

open access: yes
Nelegālās izcelsmes tabakas izstrādājumi veido Latvijā teju sesto daļu no tabakas patēriņa, tādā veidā radot zaudējumus valsts budžetam. Valsts policijas izņemtā tabaka un tabakas izstrādājumi nonāk Valsts tiesu ekspertīžu birojā, kur veic tabakas un tās
Bērziņa, Sanda Astra
core  

Arabidopsis DNA repair mutants can integrate Agrobacterium T‐DNA into the plant genome

open access: yesNew Phytologist, EarlyView.
Transient (a) and stable (b) transformation of Arabidopsis thaliana DNA repair/recombination mutants grown under normal light conditions. Summary Agrobacterium transfer DNA (T‐DNA) integration is mediated by plant DNA repair proteins of various nonhomologous end‐joining (NHEJ) pathways.
Lan‐Ying Lee   +7 more
wiley   +1 more source

The GSK3/SHAGGY‐like OsGSK3 phosphorylates and inhibits phase separation of OsFCA at Ser‐43 and Ser‐45 to regulate brassinosteroid signaling and rice architecture

open access: yesNew Phytologist, EarlyView.
A working model for GLYCOGEN SYNTHASE KINASE 3‐mediated phosphorylation of FLOWERING CONTROL LOCUS A in the regulation of brassinosteroid signaling and rice growth and development. Summary Brassinosteroid (BR) signaling plays a critical role in rice (Oryza sativa L.) grain development.
Jiaqi Zhang   +14 more
wiley   +1 more source

Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants

open access: yesNew Phytologist, EarlyView.
Potyvirus turnip mosaic virus induces neutral lipid accumulation and lipid droplet biogenesis in infected leaves for its own benefit. Summary Lipid droplets (LDs), which are dedicated to storing neutral lipids (NLs), are dynamic organelles involved in numerous other functions, including membrane remodeling during abiotic stress.
Léna Jambou   +16 more
wiley   +1 more source

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