Results 111 to 120 of about 44,886 (288)

Improving transient protein expression in agroinfiltrated Nicotiana benthamiana

New Phytologist
SummaryAgroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen‐derived effectors; transcript
Beritza, K, Watts, EC, van der Hoorn, RAL   +2 more
openaire   +3 more sources

Fel d 1‐Expressing Plant‐Derived Bioparticle: A Novel Treatment for Cat Allergy

Allergy, EarlyView.
This study investigates the allergenicity and immunogenicity profile of a novel plant‐derived Fel d 1 bioparticle (eBP). Fel d 1 eBP promotes immune tolerance by redirecting allergic Th2 responses toward Th1 and IL‐10–producing regulatory pathways, including Bregs.
Janice A. Layhadi, Liliana Cifuentes Gutierrez, Sean T. Keane, William Fulton, Nichell A. Samson, Lily Y. D. Wu, Paulina Filipaviciute, Prista Hikmawati, Oleksandra Fedina, Ana Jimenez‐Gil, Stephen R. Durham, Guy Scadding, Guy Tropper, Louis‐Philippe Vézina, Patrick Colin, Ronald van Ree, Mohamed H. Shamji   +16 more
wiley   +1 more source

Retardation of the Calvin Cycle Contributes to the Reduced CO2 Assimilation Ability of Rice Stripe Virus-Infected N. benthamiana and Suppresses Viral Infection

Frontiers in Microbiology, 2019
Rice stripe virus (RSV) is naturally transmitted by the small brown planthopper and infects plants of the family Poaceae. Under laboratory conditions, RSV can infect Nicotiana benthamiana by mechanical inoculation, providing a useful system to study RSV ...
Ji’an Bi, Ji’an Bi, Ji’an Bi, Yong Yang, Binghua Chen, Jinping Zhao, Zhuo Chen, Baoan Song, Jianping Chen, Jianping Chen, Fei Yan, Fei Yan   +11 more
doaj   +1 more source

A 212-nt long RNA structure in the Tobacco necrosis virus-D RNA genome is resistant to Xrn degradation [PDF]

, 2019
Plus-strand RNA viruses can accumulate viral RNA degradation products during infections. Some of these decay intermediates are generated by the cytosolic 5′-to-3′ exoribonuclease Xrn1 (mammals and yeast) or Xrn4 (plants) and are formed when the enzyme ...
Chaminda, Gunawardene D., Newburn, Laura R., White, K. Andrew   +2 more
core   +1 more source

Simultaneous modification of Thermotoga maritima encapsulin subunits to produce multivalent nanoparticle vaccines for non‐typhoidal Salmonella enterica

The FEBS Journal, EarlyView.
This study investigates the modularity of encapsulin—a protein nanoparticle—by simultaneously inserting up to four distinct Salmonella epitopes within the encapsulin subunit. The encapsulin variants were transiently expressed in plants where they assembled into nanoparticles; however, their accumulation and stability varied depending on the ...
Carly A. Charron, Angelo Kaldis, Shabnam Shamriz, Moussa S. Diarra, Christopher P. Garnham, Rima Menassa   +5 more
wiley   +1 more source

Detection of Poly (A) RNA in Mesophyll Cells of Nicotiana benthamiana Using in situ Hybridization

Bio-Protocol, 2015
Export of transcribed mRNAs from nucleoplasm to cytosol is an essential process for the translation of genes into proteins. This process is tightly regulated by nuclear pores, composed of about 30 nucleoporin proteins (Nups). Whether or not the mRNAs are
Yuri Mizuno, Daigo Takemoto
doaj   +1 more source

Drivers of strigolactone diversity: P450s in strigolactone biosynthesis

Journal of Integrative Plant Biology, EarlyView.
This review summarizes the discovery and functional identification of cytochrome P450 in strigolactone biosynthesis, classifies and summarizes the members discovered so far, clarifies their biological significance, discusses the technology of strigolactone synthesis research, and finally describes some problems in strigolactone research and potential ...
Changbin Niu, Harro J. Bouwmeester, Changsheng Li   +2 more
wiley   +1 more source

Nitric Oxide as a Partner of Reactive Oxygen Species Participates in Disease Resistance to Necrotrophic Pathogen Botrytis cinerea in Nicotiana benthamiana

Molecular Plant-Microbe Interactions, 2009
Nitric oxide (NO) is an essential regulatory molecule in plant immunity in synergy with reactive oxygen species (ROS). However, little is known about the role of NO in disease resistance to necrotrophic pathogens.
Shuta Asai, Hirofumi Yoshioka
doaj   +1 more source

Salicylic acid treatment and expression of an RNA-dependent RNA polymerase 1 transgene inhibit lethal symptoms and meristem invasion during tobacco mosaic virus infection in Nicotiana benthamiana. [PDF]

, 2016
BACKGROUND: Host RNA-dependent RNA polymerases (RDRs) 1 and 6 contribute to antiviral RNA silencing in plants. RDR6 is constitutively expressed and was previously shown to limit invasion of Nicotiana benthamiana meristem tissue by potato virus X and ...
Carr, John P, Chaluvadi, Srinivasa Rao, Dobson, Elizabeth A, Fu, Shih-Feng, Garland, Laura, Lee, Wing-Sham, Lewsey, Mathew G, Li, Zheng, Murphy, Alex M, Nelson, Richard S   +9 more
core   +2 more sources

Salt glands in exo‐recretohalophytes: Development, physiological functions, and prospects for improving crop salt tolerance

Journal of Integrative Plant Biology, EarlyView.
This review examines salt glands in exo‐recretohalophytes, in which epidermal stem cells differentiate into unicellular, bicellular, or multicellular salt glands. Salt ions are transported to the leaves via the transpiration stream and enter salt glands through symplastic and apoplastic pathways. Finally, salt glands actively secrete salt ions from the
Limin Wang, Junyan Xie, Yiping Zou, Chunliang Yao, Hai Fan, Chenqi Shen, Wenyan Zhou, Jingran Qin, Xinke Zhang, Baoshan Wang, Jian Zhang, Guoliang Han   +11 more
wiley   +1 more source